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PXD001065 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCompartment resolved reference map from highly purified naive, activated, effector and memory CD8+ cells
DescriptionDifferentiation of CD8+ T lymphocytes into effector and memory cells is key for an adequate immune response and relies on complex interplay of pathways that convey signals from cell surface to nucleus. In this study, we fractionated four CD8+ T cell subtypes; naïve, recently activated effector, effector and memory cells into membrane, cytosol, soluble nucleus, chromatin-bound and cytoskeleton compartments. Using LC-MS/MS analysis, identified peptides were matched to human peptides/proteins (SwissProt). Compartment fractionation and gel-LC-MS separation identified 2399 proteins in total. Among these 735 were detected in all five, 241 in four, 257 in three, 368 in two and 798 found in only one fraction. Comparison between the two most different subsets, naïve and effector, yielded 146 significantly regulated proteins.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJanina Tomm
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-06-16 02:39:21ID requested
12015-02-04 04:30:18announced
Publication List
Zanker D, Otto W, Chen W, von Bergen M, Tomm JM, murine immune cells. Proteomics, 15(11):1808-12(2015) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: murine, CD8+, LC-MS/MS
Contact List
Martin von Bergen
contact affiliationDepartment of Proteomics, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318 Leipzig, Germany
contact emailmartin.vonbergen@ufz.de
lab head
Janina Tomm
contact affiliationHelmholtzzentrum für Umweltforschung - UFZ
contact emailjanina.tomm@ufz.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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