PXD000111

PXD000111 is an original dataset announced via ProteomeXchange.

Title	Mycobacterium tuberculosis culture filtrate LC-MS/MS
Description	Triplicate culture filtrate samples of normal (log phase) and PBS cultured (starvation) M. tuberculosis were separated by SDS-PAGE, and 10 bands were excised from each lane after staining with Coomassie R-250. In-gel trypsin digestion was performed on gel bands followed by nanoLC. Eluting peptides were then analyzed on an LTQ FT instrument, and the resulting raw files were searched with Sequest (part of Proteome Discoverer) against an M. tuberculosis H37Rv database (downloaded from the Sanger Centre). The final combined dataset comprised 12,399 unique peptides derived from 1,362 proteins, each identified by a minimum of two distinct peptides.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:16:48.902.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
Reprocessed datasets that are derived from this dataset	RPXD000650
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ida Rosenkrands
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2012-12-18 04:25:37	ID requested
1	2013-01-24 03:55:52	announced
1	2014-07-25 00:47:59	announced
2	2013-02-11 02:26:23	announced	Add reference
2	2017-06-21 00:30:34	announced	Updated project metadata.
3	2017-10-24 00:11:24	announced	Updated project metadata.
⏵ 4	2024-10-22 04:16:49	announced	2024-10-22: Updated project metadata.

Albrethsen J, Agner J, Piersma SR, H, ø, jrup P, Pham TV, Weldingh K, Jimenez CR, Andersen P, Rosenkrands I, Proteomic profiling of Mycobacterium tuberculosis identifies nutrient-starvation-responsive toxin-antitoxin systems. Mol Cell Proteomics, 12(5):1180-91(2013) [pubmed]

10.1074/mcp.M112.018846;

submitter keyword: Tuberculosis, culture filtrate, LC-MS/MS

Ida Rosenkrands
contact affiliation	Infectious Disease Immunology
contact email	idr@ssi.dk
lab head
Ida Rosenkrands
contact affiliation	Infectious Disease Immunology
contact email	idr@ssi.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2013/01/PXD000111

PRIDE project URI

• PRIDE
  1. PXD000111
     1. Label: PRIDE project
     2. Name: Mycobacterium tuberculosis culture filtrate LC-MS/MS