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RPXD034723

RPXD034723 is container for one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.

Reanalysis Container Summary

Title	JPST000096-1 Beige adipocyte phosphoproteomics [Reanalysis: JPST000096]
Description	Catecholamines promote lipolysis both in brown and white adipocytes, whereas the same stimuli preferentially activate thermogenesis in brown adipocytes. Molecular mechanisms for the adipose-selective activation of thermogenesis remain poorly understood. Here, we employed quantitative phosphoproteomics to map global and temporal phosphorylation profiles in brown, beige, and white adipocytes under Î²3-adrenenoceptor activation and identified kinases responsible for the adipose-selective phosphorylation profiles. We found that casein kinase2 (CK2) activity is preferentially higher in white adipocytes than brown/beige adipocytes. Genetic or pharmacological blockade of CK2 in white adipocytes activates the thermogenic program in response to cAMP stimuli. Such activation is largely through reduced CK2-mediated phosphorylation of class I HDACs. Notably, inhibition of CK2 promotes beige adipocyte biogenesis and leads to an increase in whole-body energy expenditure and ameliorates diet-induced obesity and insulin resistance. These results indicate that CK2 is a plausible target to rewire the Î²3-adrenenoceptor signaling cascade that promotes thermogenesis in adipocytes. [Original project description]
HostingRepository	jPOST
AnnounceDate	2023-03-11
AnnouncementXML	Submission_2023-03-21_03:44:39.860.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/RPXD034723
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Reanalysis of JPST000096
RepositorySupport	Supported dataset by repository
PrimarySubmitter	jPOST reanalysis
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	Q Exactive

Dataset History

Reanalysis	Revision	Datetime	Status	Title / ChangeLog Entry
	0	2022-06-20 17:55:19	ID requested
0	1	2023-03-11 06:53:50	announced	JPST000096-1 Beige adipocyte phosphoproteomics [Reanalysis: JPST000096]
0	2	2023-03-21 03:44:40	announced	JPST000096-1 Beige adipocyte phosphoproteomics [Reanalysis: JPST000096] 2023-03-21: Updated AnnounceDate.

Publication List

no publication

no publication

Keyword List

submitter keyword: reanalysis, Beige adipocyte, phosphoproteomics

submitter keyword: reanalysis, Beige adipocyte, phosphoproteomics

Contact List

Yasushi Ishihama
lab head
jPOST reanalysis
contact affiliation	jPOST
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001720/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001720/