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RPXD034664

RPXD034664 is container for one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.

Reanalysis Container Summary
TitleJPST000115-1 Identification of ubiquitylation sites by K0-Ub [Reanalysis: JPST000115]
DescriptionWe have now developed a method for the efficient identification of ubiquitylation sites in target proteins with the use of an engineered form of ubiquitin (K0-Ub), in which all seven lysine residues are replaced with arginine. K0-Ub is covalently attached to lysine residues of target proteins via an isopeptide bond, but further formation of a polyubiquitin chain does not occur on K0-Ub. We identified a total of 1392 ubiquitylation sites of 794 proteins from HEK293T cells. [Original project description]
HostingRepositoryjPOST
AnnounceDate2023-03-25
AnnouncementXMLSubmission_2023-03-25_06:08:46.595.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/RPXD034664
ReviewLevelNon peer-reviewed dataset
DatasetOriginReanalysis of JPST000115
RepositorySupportSupported dataset by repository
PrimarySubmitterjPOST reanalysis
SpeciesList scientific name: HEK293T; NCBI TaxID: BTO:0002181;
ModificationListS-carboxamidomethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Gln); L-methionine sulfoxide; unknown modification
InstrumentLTQ Orbitrap
Dataset History
ReanalysisRevisionDatetimeStatusTitle / ChangeLog Entry
02022-06-19 17:27:18ID requested
012023-03-25 06:08:47announcedJPST000115-1 Identification of ubiquitylation sites by K0-Ub [Reanalysis: JPST000115]
Publication List
no publication
Keyword List
submitter keyword: reanalysis, Lysine-less ubiquitin
Contact List
Yasushi Ishihama
lab head
jPOST reanalysis
contact affiliationjPOST
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001682/