⮝ Full datasets listing
PXD073797
PXD073797 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Development of PolyHis-Targeting PROTAC Degraders |
| Description | Targeted protein degradation (TPD) via proteolysis targeting chimeras (PROTACs) enables selective removal of proteins of interest (POIs) by hijacking the ubiquitin-proteasome system (UPS). However, broad application is constrained by the availability of high-quality target ligands, which remain scarce for much of the human proteome, limiting assessment of POIs for UPS-mediated degradation. To address this challenge, we developed polyhistidine-targeting PROTACs (polyHisTACs) by conjugating a nickel-nitrilotriacetic acid (Ni2+-NTA) headgroup to ligands of VHL or CRBN, thereby recruiting these E3 ligase complexes to polyHis-tagged POIs. As expected, polyHisTACs effectively degraded CRISPR-engineered, endogenously polyHis-tagged BRD4 and also induced robust degradation of an exogenously expressed polyHis-tagged RNA-binding protein, PSPC1, a target that is typically considered undruggable. In summary, polyHisTACs overcome key limitations of existing tag-based degrader systems by leveraging a minimal, easily implemented polyHis tag. This platform provides a versatile, reliable way to evaluate UPS-mediated degradability in the absence of target-specific ligands and serves as a practical tool for acute POI depletion in basic research. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-01-29 |
| AnnouncementXML | Submission_2026-01-29_13:14:13.544.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Susan T. Weintraub |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-29 12:58:02 | ID requested | |
| ⏵ 1 | 2026-01-29 13:14:14 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: PROTAC, targeted protein degradation, polyhistidine, HiBiT, DIA-MS, DatasetType:Proteomics |
Contact List
| Dongwen Lyu (Lv), Ph.D., M.S. | |
|---|---|
| contact affiliation | Medical College of Georgia at Augusta University |
| contact email | dlyu@augusta.edu |
| lab head | |
| Susan T. Weintraub | |
| contact affiliation | UT Health Science Center San Antonio |
| contact email | weintraub@uthscsa.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000100649/ |
