⮝ Full datasets listing
PXD072811
PXD072811 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of large extracellular vesicles released by apoptotic and pyroptotic cells |
| Description | Extracellular vesicles (EVs) are emerging key factors maintaining cellular homeostasis, critical mediators of intercellular communication, potential biomarkers and therapeutic tools. While small EVs have been extensively characterized, the molecular signatures of large EVs (including those generated during regulated cell death pathways) remain poorly defined. Here, we investigated the characteristics of large EVs released during apoptosis and pyroptosis by human monocytic cell lines (THP-1 and U937). Apoptosis was induced by staurosporine and blocked with the pan-caspase inhibitor Q-VD-OPh, whereas pyroptosis was triggered by LPS/nigericin and inhibited with a selective NLRP3 inhibitor. We found that both forms of regulated cell death markedly enhanced the release of large EVs. Both apoptotic and pyroptotic large EVs showed increased Annexin V binding and decreased CD9 expression as compared to those released by healthy living cells. Apoptosis- and pyroptosis-derived large EVs exhibited distinct proteomic profiles. Pyroptotic large EVs were shown to contain interacting protein networks of RNA binding proteins and chromatin-associated proteins many of which are known as established damage associated molecular patterns or alarmins. On the other hand, we found that a subpopulation of apoptotic large EVs was characterized by the presence of dsDNA, and active caspase-3/7. Together, our data shed light on the specific protein content of large EVs released by cells undergoing apoptosis and pyroptosis. This study identifies candidate markers of large EVs released by dying cells and may enhance our understanding of the role of EVs in cell death. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-01-13 |
| AnnouncementXML | Submission_2026-01-13_02:04:14.325.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Eva Hunyadi-Gulyas |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-08 04:16:47 | ID requested | |
| ⏵ 1 | 2026-01-13 02:04:14 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: large extracellular vesicle, apoptosis, pyroptosis, LC-MSMS, TMTpro 16plex, DatasetType:Proteomics |
Contact List
| Edit Buzas | |
|---|---|
| contact affiliation | Institute of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest |
| contact email | buzas.edit@semmelweis.hu |
| lab head | |
| Eva Hunyadi-Gulyas | |
| contact affiliation | BRC |
| contact email | gulyas.eva@brc.hu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100407/ |
