PXD072555

PXD072555 is an original dataset announced via ProteomeXchange.

Title	Identify the Therapeutic Role and Potential Mechanism of α-Cyperone in diminished ovarian reserve Based on Network Pharmacology, Molecular docking, Lip-MS and Experimental Validation
Description	Research hypothesis: α-Cyperone regulates the hormone metabolism/steroideogenesis pathway by directly binding to the key proteins of ovarian granulosa cells (KGN), thereby alleviating DOR. In vitro, limited proteolysis–mass spectrometry (LiP–MS) has provided a way to observe changes in both protein abundances and structures on a proteome-wide scale. We extracted whole-cell protein samples from CTX-induced KGN cells, divided the samples into two aliquots, and one sample was incubated with AC for 24h. Two samples were treated with proteinase K (PK), and standard sample preparation was performed on two duplicate samples. All peptides were subjected to liquid chromatography-tandem MS (LC-MS/MS) in data independent acquisition (DIA) mode for DIA analysis .
HostingRepository	iProX
AnnounceDate	2025-12-31
AnnouncementXML	Submission_2025-12-31_00:54:47.534.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jingwen Guo
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-12-31 00:54:34	ID requested
⏵ 1	2025-12-31 00:54:48	announced

Dataset with its publication pending

submitter keyword: α-Cyperone, Diminished Ovarian Reserve, Network pharmacology, Molecular docking, Experimental validation, Limited proteolysis–mass spectrometry

Jingwen Guo
contact affiliation	General Hospital of Ningxia Medical University
contact email	2718148023@qq.com
lab head
Jingwen Guo
contact affiliation	General Hospital of Ningxia Medical University
contact email	2718148023@qq.com
dataset submitter

iProX dataset URI