PXD071103

PXD071103 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of spermathecal fluid and seminal plasma reveals the mechanism of sperm storage in Amphioctopus fangsiao
Description	The development of males and females of the cephalopod Amphioctopus fangsiao is asyn-chronous. The male produces sperm after maturity for storage in a spermatophore prior to mating. After mating, the sperm enters the female spermatheca for storage until ovulation occurs, a period that lasts for 8 months. This is a biologically uncommona rare phenomenon, because sperm cells generally fail to maintain their ability to fertilize for a long time after being ejaculated However, the molecular mechanisms about this phenomenon are still not clear. Sperm cells are stored in the male spermatophore and the female spermatheca, each of which provides a suitable environment. To determine the molecular basis of the sperm stor-age mechanisms in A. fangsiao, protein profiles from spermathecal fluid and seminal plasma separately were characterized using mass spectrometry-based proteomics. The antioxidant enzymes superoxide dismutase (SOD), glutathione S-transferase (GST), and Thioredoxin (Trx) and the glycolytic enzymes lactate dehydrogenase (LDH), hexokinase (HK), pyruvate dehydrogenase kinase (PDK), and ATP synthase were significantly enriched in the sperma-thecal fluid. Catalase (CAT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), tri-osephosphate isomerase (TIM), and phosphoglycerate kinase (PGK) were significantly en-riched in the seminal plasma. The antimicrobial proteins transforming growth factor beta regulator 1 (TBRG1) and interleukin enhancer binding factor 2 (ILF2) and the extracellular matrix-related proteins transforming growth factor beta induced protein (TGFBIp) and thrombospondin type-1 domain-containing protein 4 (THSD4) were also significantly ex-pressed in the spermathecal fluid. These proteins may be crucial for successful long-term sperm storage. We measured the activities of four antioxidant enzymes based on the proteomic results, with the enzyme activity results showing consistency with the proteomic data. This study enhances our understanding of the sperm storage ability of A. fangsiao
HostingRepository	iProX
AnnounceDate	2025-11-20
AnnouncementXML	Submission_2025-11-23_17:01:58.606.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiantao Yao
SpeciesList	scientific name: Amphioctopus fangsiao; NCBI TaxID: 515817;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-23 17:01:46	ID requested
⏵ 1	2025-11-23 17:01:59	announced

Dataset with its publication pending

submitter keyword: Amphioctopus fangsiao

Spermathecal fluid

Seminal plasma

Sperm storage

Proteome analysis

Yanwei Feng
contact affiliation	Ludong University
contact email	fywzxm1228@163.com
lab head
Jiantao Yao
contact affiliation	Ludong University
contact email	yjtsober123@163.com
dataset submitter

iProX dataset URI