PXD070823

PXD070823 is an original dataset announced via ProteomeXchange.

Title	Photodynamic therapy with a novel photosensitizer inhibits BLM-induced pulmonary fibrosis in mice via MRC1-mediated pathway
Description	For proteomic analysis, the middle lobe of right lung was dissected from each mouse. Tissues from individual mice were processed separately (not pooled) to account for biological variability, with n=3 per group used for this analysis. Lung tissue were homogenized in 600 μL of ice-cold lysis buffer (8 M urea supplemented with protease inhibitors). The homogenates were subjected to intermittent sonication (1 s on, 2 s off, for a total of 120 s) and subsequently centrifuged at 14,000 ×g for 20 min at 4°C to collect the supernatant. Protein concentration was determined using a bicinchoninic acid (BCA) assay. Protein quality and integrity were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Further separation was performed using a RIGOL L-3000 rapid resolution high-performance liquid chromatography (RP-HPLC) system following the manufacturer's instructions.
HostingRepository	iProX
AnnounceDate	2025-11-16
AnnouncementXML	Submission_2025-11-17_00:57:05.723.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yumei Rong
SpeciesList	scientific name: Rattus rattus; NCBI TaxID: 10117;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-17 00:56:28	ID requested
⏵ 1	2025-11-17 00:57:06	announced

Dataset with its publication pending

submitter keyword: Mrc1

Yumei Rong
contact affiliation	Tianjin University Central Hospital
contact email	yumeirong0803@163.com
lab head
Yumei Rong
contact affiliation	Tianjin University Central Hospital
contact email	yumeirong0803@163.com
dataset submitter

iProX dataset URI