⮝ Full datasets listing
PXD070658
PXD070658 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Commensal derived short chain fatty acids disrupt Staphylococcus aureus lipid membrane homeostasis |
| Description | Quantitative, tandem mass tag (TMT) based proteomics for Staphylococcus aureus JE2 grown in LB alone or LB supplemented with the commensal short-chain fatty acids propionate or butyrate. Mid-log cells (OD600 0.3) were lysed (urea/thiourea/TEAB buffer), reduced/alkylated, barocycled, digested, TMT-labeled, and analyzed on an Orbitrap Eclipse using HCD. Raw files and search results (Proteome Discoverer; UniProt S. aureus pan-proteome UP000008816 plus common contaminants) are included. The dataset supports the mBio study Commensal-derived short-chain fatty acids disrupt membrane lipid homeostasis in Staphylococcus aureus (Fletcher et al.) and enables assessment of global protein abundance changes elicited by SCFAs that converge on lipid/branched-chain fatty acid metabolism, cell envelope homeostasis, and virulence regulation. Differential proteins highlighted in the manuscript include induction of acetolactate synthase components (BudA/AlsS) and NanA in propionate, increased dihydropicolinate synthases (DapA/B/H) in butyrate, and decreased RecR and NrdI in both conditions. These data provide a resource to explore SCFA-driven remodeling of the S. aureus proteome linked to membrane physiology and antimicrobial sensitivity. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-11-12 |
| AnnouncementXML | Submission_2025-11-12_19:17:19.857.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ryan Hunter |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-11-12 18:08:42 | ID requested | |
| ⏵ 1 | 2025-11-12 19:17:20 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Staphylococcus aureus, Propionate, butyrate, short chain fatty acids, DatasetType:Proteomics |
Contact List
| Ryan Hunter | |
|---|---|
| contact affiliation | University at Buffalo |
| contact email | rhunter2@buffalo.edu |
| lab head | |
| Ryan Hunter | |
| contact affiliation | University at Buffalo |
| contact email | rhunter2@buffalo.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099871/ |
