PXD070369

PXD070369 is an original dataset announced via ProteomeXchange.

Title	HMGN1 and HMGN2 are recruited to acetylated and histone variant H2A.Z-containing nucleosomes to regulate chromatin state and transcription
Description	The High Mobility Group Nucleosome-binding (HMGN) proteins are small abundant nuclear proteins that directly bind nucleosomes and constitute a major component of chromatin. HMGN proteins specifically localize to enhancers and actively transcribed genes across the genome, however the roles of HMGN proteins in regulating chromatin structure and transcription remain poorly understood. To investigate the function of HMGN proteins on chromatin, we engineered mouse embryonic stem cells (mESCs) lacking HMGN1 and/or HMGN2 (Hmgn1-/- mESCs, Hmgn2-/- mESCs, and Hmgn1-/-Hmgn2-/- mESCs) and performed mass spectrometry-based quantification of a panel of histone residue states to quantify the levels of specific histone modifications in WT and Hmgn1-/-Hmgn2-/- mESCs.
HostingRepository	PRIDE
AnnounceDate	2025-11-22
AnnouncementXML	Submission_2025-11-21_18:20:42.743.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Riya Gohil
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monomethylated residue; trimethylated residue; acetylated residue; dimethylated residue
Instrument	Dionex instrument model; TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-05 11:04:32	ID requested
⏵ 1	2025-11-21 18:20:43	announced

Dataset with its publication pending

submitter keyword: E1A binding protein p300 (P300), nucleosome, epigenetics, genome structure, gene expression,histone acetylation, chromatin, high mobility group proteins

Jill Mae Dowen
contact affiliation	Associate Professor, Department of Biochemistry & Biophysics, University of North Carolina at Chapel Hill, USA
contact email	jilldowen@unc.edu
lab head
Riya Gohil
contact affiliation	University of North Carolina at Chapel Hill
contact email	riyagohil@unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD070369

PRIDE project URI

• PRIDE
  1. PXD070369
     1. Label: PRIDE project
     2. Name: HMGN1 and HMGN2 are recruited to acetylated and histone variant H2A.Z-containing nucleosomes to regulate chromatin state and transcription