PXD070339

PXD070339 is an original dataset announced via ProteomeXchange.

Title	Enhanced STEAP4 Ubiquitination in Obesity: Insights from Combined Proteome and Ubiquitylome Analysis of Visceral Adipose Tissue
Description	Obesity remains a worldwide health issue, with visceral adipose tissue as a leading driver of this pathology. As the executors of biological functions in living cells, proteins have their activity regulated by diverse post-translational modifications, including ubiquitination. However, obesity-related changes in ubiquitination of VAT proteins are still poorly understood. Here, we obtained the global proteomic and ubiquitylomic data of epididymal VAT from lean and obese male mice by mass spectrometry. Our proteomic analyses revealed significant changes of metabolic pathways involved in fatty acid, acyl-CoA and branched chain amino acids metabolism in obese VAT. Intriguingly, a comparative analysis of proteomic and ubiquitylomic data highlighted a discordance in the quantity changes of certain proteins and their ubiquitination levels. Notably, STEAP4 exhibited a markedly reduced protein level coupled with an enhanced K48-linked ubiquitination, suggesting a potential role for ubiquitination-mediated proteasome degradation in VAT dysfunction. Further in vitro experiments revealed that knockdown of STEAP4 in adipocytes impaired mitochondrial function of 3T3-L1 adipocytes. Collectively, this study introduces the first combined proteomic and ubiquitylomic examination of murine VAT, offering novel insights and potential therapeutic targets for obesity.
HostingRepository	PRIDE
AnnounceDate	2025-11-26
AnnouncementXML	Submission_2025-11-25_18:21:03.074.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhao Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	ubiquitinylated lysine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-04 20:47:52	ID requested
⏵ 1	2025-11-25 18:21:03	announced

Dataset with its publication pending

submitter keyword: None

Yuhao Li
contact affiliation	Department of Immunology, College of Basic Medicine, Army Medical University (Third Military Medical University), Chongqing 400038, China.
contact email	liyuhao616@163.com
lab head
Yuhao Li
contact affiliation	Institute of Immunology PLA, Army Medical University (Third Military Medical University), Chongqing 400038, China
contact email	liyuhao616@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070339
     1. Label: PRIDE project
     2. Name: Enhanced STEAP4 Ubiquitination in Obesity: Insights from Combined Proteome and Ubiquitylome Analysis of Visceral Adipose Tissue