PXD070271

PXD070271 is an original dataset announced via ProteomeXchange.

Title	CeD-active Peptides in Gluten-Free Beer
Description	Gluten-free barley beers may cause symptoms in celiac disease (CeD) patients due to CeD-active peptides from partially hydrolyzed gluten. Accurate quantitation of such peptides is essential to improve analytical methods for assessing CeD immunogenic activity and ensuring consumer safety, as routine methods like R5 competitive enzyme-linked immunosorbent assays (R5c ELISA) may miss small yet harmful peptides. This study used targeted nano-liquid chromatography-tandem mass spectrometry (nanoLC MS/MS) with stable isotope dilution assay to quantitate seven CeD-active peptides with complete immunogenic epitopes in 19 gluten-free and four regular or carbohydrate-reduced beers. The selection of CeD-active peptides was made based on their previous identification in the analyzed beers. The beers were subjected to single-pot solid-phase-enhanced sample preparation and chymotryptic hydrolysis. All peptides were under the limit of quantitation in 13 commercially available gluten-free beers. In the remaining six, the total peptide concentrations ranged from 14–806 ng/mL, while non-gluten-free beers contained 967–3089 ng/mL. Peptide concentrations correlated with the gluten content measured by R5c ELISA, and lot-to-lot variations were observed. These findings support the need for a more comprehensive risk assessment of products containing partially hydrolyzed gluten.
HostingRepository	PanoramaPublic
AnnounceDate	2025-11-03
AnnouncementXML	Submission_2025-11-03_19:32:10.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eleonora Tissen
SpeciesList	scientific name: Triticum; NCBI TaxID: 4564; scientific name: Hordeum vulgare; NCBI TaxID: 4513;
ModificationList	Label:13C(9)15N(1); Label:13C(5)15N(1)
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-11-03 19:27:46	ID requested
⏵ 1	2025-11-03 19:32:10	announced

Tissen E, Geisslitz S, Scherf KA. Absolute quantitation of celiac disease-active gluten peptides in gluten-free barley beer by targeted nanoLC-MS/MS. Food Res Int [Internet]. 2025;117703. Available from: https://www.sciencedirect.com/science/article/pii/S0963996925020411

submitter keyword: brewing, immunogenic epitopes, partially hydrolyzed gluten, stable isotope dilution assay, targeted proteomics

Katharina Scherf
contact affiliation	TU Munich, TUM School of Life Sciences, Professorship of Food Biopolymer Systems, Freising, Germany; Leibniz Institute for Food Systems Biology at the TU Munich, Freising, Germany
contact email	k.scherf.leibniz-lsb@tum.de
lab head
Eleonora Tissen
contact affiliation	TU Munich, TUM School of Life Sciences, Professorship of Food Biopolymer Systems, Freising, Germany; Leibniz Institute for Food Systems Biology at the TU Munich, Freising, Germany
contact email	e.tissen.leibniz-lsb@tum.de
dataset submitter

Panorama Public dataset URI