PXD070180

PXD070180 is an original dataset announced via ProteomeXchange.

Title	An abundant membrane protein in the NCAM superfamily is required for epithelial organization in sea anemone embryos
Description	Assembly of cells into epithelial layers marks the early steps of tissue organization and embryonic development in most animals. Although several conserved proteins are known to be essential for epithelialization in bilaterians, it is unclear whether these are sufficient to drive epithelial organization in all metazoans. Using proteomics and knockdown approaches in embryos of the early-branching sea anemone Nematostella vectensis, we identified the neural cell adhesion molecule NCAM2 to be essential for organization of the primary epithelial layer. NCAM2 belongs to the immunoglobulin family of cell adhesion molecules with roles in inter-cellular adhesion and signaling. In this work, we show that NCAM2 is enriched at the apical cell junctions and is required for Nematostella epithelial organization. Importantly, embryos depleted for NCAM2 fail to gastrulate. Both whole-embryo germ layer patterning and epithelial integrity required for gastrulation are impaired. Our results highlight the importance of an NCAM family-protein in the organization of primary epithelia and early embryonic development.
HostingRepository	PRIDE
AnnounceDate	2025-12-04
AnnouncementXML	Submission_2025-12-04_10:39:55.168.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katerina Ragkousi
SpeciesList	scientific name: Nematostella vectensis; NCBI TaxID: NEWT:45351;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-31 20:07:58	ID requested
⏵ 1	2025-12-04 10:39:55	announced

Dataset with its publication pending

submitter keyword: None

Katerina Ragkousi
contact affiliation	Amherst College
contact email	kragkousi@amherst.edu
lab head
Katerina Ragkousi
contact affiliation	Amherst College
contact email	kragkousi@amherst.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD070180
     1. Label: PRIDE project
     2. Name: An abundant membrane protein in the NCAM superfamily is required for epithelial organization in sea anemone embryos