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PXD070070
PXD070070 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mechanism of Hepatotoxicity Induced by Ethanol Extract of Emilia sonchifolia (L.) DC Revealed by Proteomics and Metabolomics |
| Description | Objective: Hepatotoxicity has been reported for Emilia sonchifolia (L.) DC (Emilia sonchifolia). The plant material is typically prepared using two extraction methods for practical application: water extraction and ethanol extraction. However, our previous research only investigated its water extract. Therefore, this study aims to systematically evaluate the hepatotoxicity and underlying mechanisms of the ethanol extract of Emilia sonchifolia, thereby providing a more comprehensive scientific basis for its rational application and safety assessment. Methods: An acute toxicity preliminary screening study was conducted by orally administering Emilia sonchifolia ethanol extract to mice at doses ranging from 0 to 33.6 g/kg/day. Based on the results of the acute toxicity test preliminary screening study, mice were divided into a control group and an Emilia sonchifolia ethanol extract group (8.6 g/kg/day) for a 14-day delayed hepatotoxicity experiment based on clinical treatment duration. At the end of the intervention, hepatic pathological changes were examined using hematoxylin-eosin staining. Enzyme-linked immunosorbent assay (ELISA) was employed to quantify the levels of alanine aminotransferase , aspartate aminotransferase, total bilirubin, direct bilirubin, total bile acids, alkaline phosphatase, and γ-glutamyl transferase in serum, as well as malondialdehyde, superoxide dismutase, and catalase in liver tissue. Proteomics and metabolomics analyses were performed to investigate the mechanisms of hepatotoxicity induced by the ethanol extract. Additionally, the mRNA expression levels of Cyp3a41a, Cyp2c29, Ugt2b1, and Hsd3b3 in mice liver tissue were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results: The acute toxicity preliminary screening study results showed that a dose of 12.0 g/kg or higher of the Emilia sonchifolia ethanol extract caused acute liver failure and death in mice. A dose of 8.6 g/kg or lower of the Emilia sonchifolia ethanol extract produced dose-dependent acute hepatotoxicity. Meanwhile, a dose of 8.6 g/kg of the Emilia sonchifolia ethanol extract induced delayed toxicities in mice. Proteomics and metabolomics results revealed that the hepatotoxicity induced by the ethanol extract of Emilia sonchifolia was associated with cholestasis and oxidative stress caused by disruptions in drug metabolism, steroid hormone biosynthesis, and primary bile acid biosynthesis. Validation experiments showed that the levels of Cyp2c29 were decreased, while the mRNA levels of Cyp3a41a, Ugt2b1, and Hsd3b3 were increased in the liver tissues of mice treated with the ethanol extract of Emilia sonchifolia. Additionally, serum levels of total bilirubin, direct bilirubin, total bile acids, alkaline phosphatase, and γ-glutamyl transferase were significantly elevated. Furthermore, in the livers of mice treated with the ethanol extract, malondialdehyde levels were increased, whereas superoxide dismutase and catalase levels were decreased. Conclusion: In summary, the ethanol extract of Emilia sonchifolia can induce hepatotoxicity in mice, and its mechanism is associated with cholestasis and oxidative stress mediated by disruptions in drug metabolism, steroid hormone biosynthesis, and primary bile acid biosynthesis. |
| HostingRepository | iProX |
| AnnounceDate | 2025-10-30 |
| AnnouncementXML | Submission_2025-10-29_20:49:32.451.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Gongzhen Chen |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-29 20:49:17 | ID requested | |
| ⏵ 1 | 2025-10-29 20:49:33 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Drug-induced liver injury, ethanol extract of Emilia sonchifolia (L.) DC, hepatotoxicity, proteomics, metabolomics |
Contact List
| Xin Liu | |
|---|---|
| contact affiliation | Guizhou University of Traditional Chinese Medicine |
| contact email | 1046977102@qq.com |
| lab head | |
| Gongzhen Chen | |
| contact affiliation | Guizhou University of Traditional Chinese Medicine |
| contact email | 591013477@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
