⮝ Full datasets listing
PXD070053
PXD070053 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic profiling of adipose-derived mesenchymal stromal cell response to novel compounds targeting the Laminin Receptor PEDF interaction |
| Description | This study characterizes the proteomic responses of adipose-derived mesenchymal stromal cells (ASC) to novel compounds that target the interaction between pigment epithelium-derived factor (PEDF) and the 37 Laminin Receptor (LR). Building on previous research, we introduced the second-generation analog, 02-09, for comparative analysis with C3, the original compound. C3 was identified through in silico screening and experimental validation, showing anti-inflammatory activity. Analog 02-09 was synthesized by modifying C3's aromatic ring, resulting in similar binding affinities to LR. Proteomic profiling of ASC under undifferentiated and chondrogenic conditions was performed using LC-MS/MS, with controls including PEDF-derived peptide P18 and Kartogenin (Krt). The distinct molecular effects of C3 and 02-09, along with their potential as anti-inflammatory therapies from prior work, suggested their multi-functional utility. Proteomic profiling of ASC, under both undifferentiated and chondrogenic differentiation conditions, was performed using LC-MS/MS, with controls including the PEDF-derived peptide P18 and the cartilage-regenerative compound Kartogenin (Krt). Both C3 and 02-09 induced significant proteomic modulation compared to controls, with enrichment in pathways related tointegrin 1 signaling, extracellular matrix (ECM) organization, and TGF- signaling. C3 uniquely activated the nuclear receptors meta-pathway,whereas 02-09 was associated with laminin interactions. Protein-protein interaction and transcription factor enrichment analyses revealed distinct and overlapping regulatory networks for LR-targeting compounds. During chondrogenic differentiation, C3 primarily enhanced proteins linked to a chondrogenic phenotype with minimal fibrogenic activity, while 02-09 showed a dual influence on both chondrogenic and fibrogenic pathways. Comparative analysis of the proteomic response to C3 or 02-09 with a single-cell RNA-sequencing dataset confirmed that C3 s modulated proteins primarily correlated with a chondrogenic differentiation phenotype, with minimal fibrogenic influence. The proteins modulated by 02-09 correlated with a dual profile (both chondrogenic and fibrogenic potential). Our results suggest that C3 has potential as a targeted chondrogenic agent, while 02-09 may serve as a multifaceted modulator of both cartilage repair and ECM protection. These findings indicate the utility of these compounds for regenerative medicine and the development of disease-modifying therapies for inflammatory and immune-related conditions. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-01-06 |
| AnnouncementXML | Submission_2026-01-06_06:57:35.996.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tiago Sobreira |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-29 17:29:05 | ID requested | |
| ⏵ 1 | 2026-01-06 06:57:36 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Adipose derived mesenchymal cells, mesenchymal stromal cells, chondrogenesis, Laminin receptor 1, DatasetType:Proteomics |
Contact List
| Marxa L Figueiredo | |
|---|---|
| contact affiliation | Department of Basic Medical Sciences, College of Veterinary Medicine, Purdue |
| contact email | mlfiguei@purdue.edu |
| lab head | |
| Tiago Sobreira | |
| contact affiliation | Covant Therapeutics |
| contact email | tiagosobreira@yahoo.com.br |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099689/ |
