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PXD070027
PXD070027 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Sec14L6 Is a Phosphoinositide Transporter That Regulates Phosphoinositide Homeostasis and Biogenesis of Lipid Droplet |
| Description | Lipid droplets (LDs) are evolutionarily conserved organelles essential for cellular metabolism. They form and grow at the endoplasmic reticulum (ER), requiring lipid transfer between these compartments, yet the underlying molecular mechanisms remain elusive. We identify Sec14L6, a unique Sec14 family member, as a lipid transporter regulating phosphoinositide (PIP) homeostasis and LD biogenesis, promoting adipogenic differentiation of mesenchymal stem cells. Sec14L6 directly binds the LD biogenesis factor ACSL3, which facilitates the association of Sec14L6 with LD surface. Furthermore, the ER membrane protein PGRMC1 recruits Sec14L6 to the ER. Targeted lipidomics revealed profound PIP dysregulation in Sec14L6-KO cells: LDs accumulated phosphoinositide-4- phosphate (PI4P) and PI(4,5)P₂, while these PIPs were reduced within the ER. In vitro assays demonstrated that Sec14L6 transports PI4P and PI(4,5)P₂. Sec14L6 knockout significantly impaired LD formation; this defect was rescued by wild-type Sec14L6, but not by lipid-transfer-deficient mutants. Our study reveals an essential role for Sec14L6 in PIP homeostasis and promotes LD biogenesis through lipid transfer between the ER and LDs. |
| HostingRepository | iProX |
| AnnounceDate | 2025-10-29 |
| AnnouncementXML | Submission_2025-10-29_02:33:14.731.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tiantian Zhou |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | acetylated residue; methionine oxidation with neutral loss of 64 Da |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-29 02:33:01 | ID requested | |
| ⏵ 1 | 2025-10-29 02:33:15 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Sec14L6 |
Contact List
| Weike Ji | |
|---|---|
| contact affiliation | Huazhong University of Science and Technology |
| contact email | Weike_Ji1985@163.com |
| lab head | |
| Tiantian Zhou | |
| contact affiliation | Huazhong University of Science and Technology |
| contact email | 18234506672@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
