⮝ Full datasets listing
PXD069947
PXD069947 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Sphingosine Kinase-2 Inhibition Increases Acetyl-CoA Carboxylase Activity |
| Description | The presence of immunosuppressive cells, including myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages (TAMs), within the tumor microenvironment (TME) represents a significant challenge in adoptive T cell therapy. Consequently, strategies designed to improve anti-tumor T cell function in vivo by mitigating this immunosuppression are essential. Here in, we demonstrate that inhibiting sphingosine kinase-2 (SphK2) mediated sphingosine-1-phosphate (S1P) generation in myeloid-derived suppressor cells (MDSCs) significantly reduces their suppressive potential and promotes their antigen presenting capacity. An S1P pull-down assay, followed by mass spectrometry-based proteomics analysis, demonstrated that S1P interacts with multiple proteins essential for metabolic processes. Both isoforms of Acetyl CoA Carboxylase (ACC1 and ACC2), which are the rate-limiting enzymes in fatty acid metabolism, exhibit binding to S1P. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-05-04 |
| AnnouncementXML | Submission_2026-05-04_21:43:29.052.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Mohamed Faisal Kassir |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-10-27 10:04:53 | ID requested | |
| ⏵ 1 | 2026-05-04 21:43:30 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: S1P, MDSCs, Acaca1, DatasetType:Proteomics |
Contact List
| Shikhar Mehrotra | |
|---|---|
| contact affiliation | Medical University of South Carolina |
| contact email | mehrotr@musc.edu |
| lab head | |
| Mohamed Faisal Kassir | |
| contact affiliation | Medical University of South Carolina |
| contact email | kassir@musc.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000099648/ |
