PXD069594

PXD069594 is an original dataset announced via ProteomeXchange.

Title	A new role for lipoproteins LpqZ and FecB in orchestrating mycobacterial cell envelope biogenesis
Description	Accounting for more deaths than any other bacterial species, Mycobacterium tuberculosis (Mtb) represents a critical threat to public health worldwide. A key factor contributing to Mtb's virulence is its unique cell envelope, which acts as a protective barrier. Among the components of this envelope, lipoproteins represent a critical but understudied group of proteins. In this study, we focused on 79 conserved putative lipoproteins, shared between Mtb and the closely related M. marinum. Leveraging the CRISPR/Cas9 gene editing system for Mycobacteria, we generated frameshift mutations, targeting one conserved lipoprotein-coding gene at a time. We found two mutants, lpqZ and fecB, that showed increased susceptibility to all tested antibiotics, pointing towards critical roles in cell envelope biogenesis. Using co-immunoprecipitation (Co-IP) followed by mass spectrometry (MS), we identified interacting partners of these two lipoproteins, indicating that they are key players in LAM and arabinogalactan synthesis, thus explaining the observed phenotypes.
HostingRepository	PRIDE
AnnounceDate	2025-12-12
AnnouncementXML	Submission_2025-12-12_02:01:56.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thang Pham
SpeciesList	scientific name: Mycobacterium marinum ATCC BAA-535; NCBI TaxID: NEWT:1348799;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-17 10:15:07	ID requested
⏵ 1	2025-12-12 02:01:57	announced

Dataset with its publication pending

submitter keyword: marinum,mycobacterium, tuberculosis, lipoarabinomannan, arabinogalactan

Dr Connie Jimenez
contact affiliation	OncoProteomics Laboratory, Dept of Medical Oncology, Amsterdam University Medical Center, Amsterdam, The Netherlands
contact email	c.jimenez@amsterdamumc.nl
lab head
Thang Pham
contact affiliation	Amsterdam UMC
contact email	t.pham@amsterdamumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD069594

PRIDE project URI

• PRIDE
  1. PXD069594
     1. Label: PRIDE project
     2. Name: A new role for lipoproteins LpqZ and FecB in orchestrating mycobacterial cell envelope biogenesis