PXD069550

PXD069550 is an original dataset announced via ProteomeXchange.

Title	BORC assemblies integrate BLOC-1 subunits to diversify endosomal trafficking functions
Description	BORC and BLOC-1, evolutionarily conserved since the last eukaryotic common ancestor, orchestrate endocytic trafficking. We show that the 3D architecture of BORC - two tetramers of intertwined α-helices with N-termini converging at the core - is conserved across species. Integrative structural approaches identified assembly-critical residues and elucidated disease mutation mechanisms. Strikingly, BORC and BLOC-1 components integrate into common complexes, with BORC-disrupting mutations also impairing BLOC-1 specific components incorporation. Crosslinking mass spectrometry analysis confirmed the in vivo presence of one BORC/BLOC-1 module. EARP subunits interact with BORC/BLOC-1 mixed complexes, suggesting that a subset of the hybrid assemblies regulates recycling. These findings challenge the view of BORC and BLOC-1 as distinct entities and instead support context-dependent assembly. We propose that dynamic, modular configurations enable specialized trafficking roles.
HostingRepository	PRIDE
AnnounceDate	2026-01-07
AnnouncementXML	Submission_2026-01-07_05:15:07.539.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leopold Kremser
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Caenorhabditis elegans; NCBI TaxID: NEWT:6239;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-10-16 13:52:43	ID requested
⏵ 1	2026-01-07 05:15:08	announced

Dataset with its publication pending

submitter keyword: recycling endosome,BORC, lysosome, BLOC-1, EARP

Lukas Alfons Huber
contact affiliation	Institute of Cell Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria
contact email	lukas.a.huber@i-med.ac.at
lab head
Leopold Kremser
contact affiliation	Medical University Innsbruck
contact email	leopold.kremser@i-med.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD069550

PRIDE project URI

• PRIDE
  1. PXD069550
     1. Label: PRIDE project
     2. Name: BORC assemblies integrate BLOC-1 subunits to diversify endosomal trafficking functions