PXD068610

PXD068610 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Development and validation of a streamlined workflow for proteomic analysis of proteins and post-translational modifications from dried blood
Description	Mitra devices were used to capture 20 uL of whole blood (heparin or EDTA) followed by extraction with 5% deoxycholate (SDC) in ammonium bicarbonate, reduction/alkylation and digestion for 2 h with TPCK-trypsin. SDC was precipitated with acid and filtered. Peptides were adjusted to 80% MeCN and 1% TFA followed by purification of N-glycopeptides using Polyhydroxyethyl A spin tips. The flow though was saved for phosphopeptide enrichment, and N-glycopeptides were eluted with 0.1% TFA. Phosphopeptides were enriched using Ti-IMAC after adjusting 300 uL of flow through to 5% TFA and 0.25 M glycolic acid, and phosphopeptides were eluted with 1% NH4OH. The unenriched digest was analyzed by microflow LC and data-independent acquisition. The N-glycoenriched fraction was analyzed using an Evosep LC and Orbitral stepped-collision energy DDA-MS/MS or Orbitrap Astral DIA-MS/MS. Analyses used Spectronaut, GlycoDecipher and FragPipe glyco-N-DIA workflow with visualization in Skyline. Other details are given in the metadata file.
HostingRepository	MassIVE
AnnounceDate	2025-09-26
AnnouncementXML	Submission_2025-09-26_19:59:51.851.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matt Foster
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Phospho
Instrument	Orbitrap Exploris 480; timsTOF Pro 2; Orbitrap Astral

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-20 21:28:33	ID requested
⏵ 1	2025-09-26 19:59:52	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: n-glycoproteome, mitra device, sepsis, DatasetType:Proteomics

submitter keyword: n-glycoproteome, mitra device, sepsis, DatasetType:Proteomics

Contact List

Matthew W. Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
lab head
Matt Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099243/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000099243/