PXD068509
PXD068509 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of SovN-associated proteins immunopurified with an anti-FLAG antibody |
| Description | Immunoprecipitated proteins on beads were washed once and resuspended in 50 mM ammonium bicarbonate, followed by the addition of 200 ng trypsin/Lys-C mix and an overnight incubation at 37ºC. The digests were reduced, alkylated, acidified, and desalted with GL-Tip SDB. Then, the eluates were evaporated and dissolved in 0.1% trifluoroacetic acid and 3% acetonitrile (ACN). LC-MS/MS analysis of the resultant peptides was performed on an EASY-nLC 1200 UHPLC connected to an Orbitrap Fusion mass spectrometer through a nanoelectrospray ion source (Thermo Fisher Scientific). The peptides were separated on a 75-µm inner diameter × 150 mm C18 reversed-phase column (Nikkyo Technos) with a linear 4-32% ACN gradient for 0-100 min followed by an increase to 80% ACN for 10 min and finally held at 80% ACN for 10 min. The mass spectrometers were operated in data-dependent acquisition mode. Raw data were directly analyzed against the UniProt database restricted to Drosophila melanogaster using Proteome Discoverer 2.5 (Thermo Fisher Scientific) with the Sequest HT search engine. Peptides were filtered at a false discovery rate of 1% using the Percolator node. Label-free quantification was performed based on intensities of precursor ions using the Precursor Ions Quantifier node. Normalization was performed such that the total sum of abundance values for each sample over all peptides was the same. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-09-19 |
| AnnouncementXML | Submission_2025-09-18_19:11:47.876.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hidetaka Kosako |
| SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
| ModificationList | L-methionine sulfoxide; Acetyl; S-carboxamidomethyl-L-cysteine |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-09-18 19:11:44 | ID requested | |
| ⏵ 1 | 2025-09-18 19:11:48 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SovN, anti-FLAG IP-MS |
Contact List
| Kensaku Murano | |
|---|---|
| lab head | |
| Hidetaka Kosako | |
| contact affiliation | Tokushima University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004084/ |
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