PXD068504

PXD068504 is an original dataset announced via ProteomeXchange.

Title	Mapping the complexity of ME/CFS: Evidence for abnormal energy metabolism, altered immune profile and vascular dysfunction
Description	Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex disorder with no known underlying mechanisms, diagnostic tools, or treatments. Multiple areas of dysfunction have been extensively studied, but rarely examined together. We recruited age- and sex-matched ME/CFS patients and healthy controls for a multi-modal study examining energy metabolism, immune profiles and plasma protein levels. Adenosine monophosphate (AMP) were higher in immune cells from MECFS patients, with a similar but non-signficant trend observed in plasma. Additionally, immune cells showed higher levels of adenosine diphosphate (ADP) and a reduced adenosine triphosphate/adenosine diphosphate (ATP/ADP) ratio. These findings imply decreased ATP generation and the presence of energy stress within the immune cell population. Adaptive immune cell populations were skewed towards less mature effector subsets of CD4+, CD8+ and γδ T cells, and proportions of CD1c+CD141- conventional DC type 2 (cDC2) and CD56lowCD16+ terminal natural killer (NK) cells were also reduced. Elevated levels of plasma proteins associated with thrombus formation and vascular reactivity may contribute to the endothelial dysfunction observed in ME/CFS patients. Using Classification and Regression Tree (CART) modelling, we identified variables from each mode of investigation with strong predictive potential for ME/CFS. Together, this study provides new insights intothe somatic symptoms and underlying biology of ME/CFS.
HostingRepository	PRIDE
AnnounceDate	2025-11-14
AnnouncementXML	Submission_2025-11-13_23:04:58.547.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shivani Krishnamurthy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-18 18:08:58	ID requested
⏵ 1	2025-11-13 23:04:59	announced

Dataset with its publication pending

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Plasma (B/D-HPP)

submitter keyword: Metabolomics, Immune dysfunction,Energy metabolism, Myalgic Encephalomyelitis/Chronic Fatigue Syndrome

Dr. Benjamin Heng
contact affiliation	Faculty of Medicine, Health and Human Sciences, MacquarieUniversity.
contact email	benjamin.heng@mq.edu.au
lab head
Shivani Krishnamurthy
contact affiliation	Macquarie University
contact email	shivani.krishnamurthy@hdr.mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD068504
     1. Label: PRIDE project
     2. Name: Mapping the complexity of ME/CFS: Evidence for abnormal energy metabolism, altered immune profile and vascular dysfunction