PXD068339

PXD068339 is an original dataset announced via ProteomeXchange.

Title	Novel biological markers associated with Alzheimer's disease were identified by integrating transcriptomic and proteomic data
Description	Alzheimer’s disease (AD) is a complex disorder with significant genetic contributions, yet only a limited number of risk loci have been conclusively identified. This research aimed to discover novel potential biomarkers for AD through multi-omics and brain pathology analysis. In this study, we investigated hippocampal molecular alterations in APP/PS1 mouse using transcriptomics and data-independent acquisition (DIA) proteomics. To further validate the involvement of differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) in AD pathology and potential drug treatment, we performed an integrative analysis incorporating pathological data and protein-protein interaction networks. We identified 263 DEGs and 448 DEPs. Integrative transcriptomic and proteomic analyses revealed five co-upregulated DEGs/DEPs and one co-downregulated DEG/DEP. Comparison of KEGG pathway enrichment between the two datasets showed significant involvement in the complement and coagulation cascade, as well as neurodegeneration-multiple diseases. Furthermore, mRNA levels of LY86, CD180, and C1QB were strongly associated with amyloid-β plaque load in the AD mouse hippocampus. Protein-protein interaction analysis suggested that APP, LY86, CD180, and C1QB could serve as potential therapeutic targets for AD. The study identified three novel AD loci (EGFL8, ERMN, and CD180), with CD180 showing association with AD at both the expression and pathological levels, highlighting their potential roles in disease progression and therapeutic intervention.
HostingRepository	iProX
AnnounceDate	2025-09-12
AnnouncementXML	Submission_2025-09-13_23:35:55.840.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shitao Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-13 23:35:44	ID requested
⏵ 1	2025-09-13 23:35:56	announced

Dataset with its publication pending

submitter keyword: Alzheimer’s disease, transcriptomics, proteomics

Shitao Wang
contact affiliation	Affiliated Fuyang People's Hospital of Anhui Medical University
contact email	wangshitaomd@163.com
lab head
Shitao Wang
contact affiliation	Affiliated Fuyang People's Hospital of Anhui Medical University
contact email	wangshitaomd@163.com
dataset submitter

iProX dataset URI