PXD068249

PXD068249 is an original dataset announced via ProteomeXchange.

Title	Plastoglobules proteome of maize
Description	Despite the inherent difficulties in isolating PGs due to their lipid-rich nature and their tight association with thylakoid membranes, coupled with the absence of a robust, established protocol specifically for maize, we successfully adapted and significantly optimized the Arabidopsis thaliana PG isolation methodology. Our refined protocol involved initial isolation of intact chloroplasts from leaves, followed by ultrasonic disruption to release PGs and membrane fragments. Subsequently, the ultrasonically disrupted samples underwent two rounds of sucrose density gradient ultracentrifugation, yielding highly purified PGs. These purified PGs were then subjected to trypsin digestion and desalting, and the resulting peptides were analyzed by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Proteomics data were acquired using Data-Independent Acquisition (DIA) technology. This rigorous approach allowed us to obtain comprehensive PGs proteomic profiles from two biological replicates of B73 inbred line leaves.
HostingRepository	iProX
AnnounceDate	2025-09-10
AnnouncementXML	Submission_2025-12-07_17:55:34.372.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yongcai Huang
SpeciesList	scientific name: Zea mays; NCBI TaxID: 4577;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-09-10 02:26:42	ID requested
⏵ 1	2025-12-07 17:55:34	announced

Dataset with its publication pending

submitter keyword: Plastoglobules, Maize, proteome

Yongcai Huang
contact affiliation	Sichuan Agricultural University
contact email	ychuang@sicau.edu.cn
lab head
Yongcai Huang
contact affiliation	Sichuan Agricultural University
contact email	ychuang@sicau.edu.cn
dataset submitter

iProX dataset URI