PXD067822

PXD067822 is an original dataset announced via ProteomeXchange.

Title	Targeting the UFL1-PARP1 axis amplifies anti-tumor immunity
Description	Cancer immunotherapies are widely used to treat various cancers but are largely ineffective against pancreatic ductal adenocarcinoma (PDAC), with the underlying mechanisms poorly understood. UFMylation, a ubiquitin-like modification, regulates diverse biological and pathological processes, yet its role in PDAC remains unclear. This study reports that UFMylation drives PDAC growth and resistance to cancer immunotherapy. Mechanistically, the E3 ligase UFL1 facilitates PARP1 UFMylation, preventing its ubiquitination and degradation. Stabilized PARP1 enhances DNA damage repair, suppresses R-loop formation, and inhibits cGAS-STING activation, thereby promoting tumor immune evasion. In contrast, inhibiting UFMylation reverses these effects, facilitating tumor infiltration of cytotoxic CD8+ T cells and improving the efficacy of anti-PD-1 immunotherapy in pancreatic tumor model. Clinically, UFL1 protein levels are positively correlated with PARP1 and inversely correlated with cGAS-STING activation and CD8+ T cell infiltration in PDAC. These findings highlight UFMylation as a promising therapeutic target to enhance immunotherapy in PDAC.
HostingRepository	iProX
AnnounceDate	2025-08-28
AnnouncementXML	Submission_2025-08-31_20:01:17.586.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuan He
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-28 01:57:36	ID requested
⏵ 1	2025-08-31 20:01:17	announced

Dataset with its publication pending

submitter keyword: PARP1, UFMylation, DNA damage, cGAS-STING, Cancer immunotherapy

Jinfang Zhang
contact affiliation	Wuhan University
contact email	jinfang_zhang@whu.edu.cn
lab head
Chuan He
contact affiliation	Wuhan University
contact email	chuan_he@whu.edu.cn
dataset submitter

iProX dataset URI