PXD067649

PXD067649 is an original dataset announced via ProteomeXchange.

Title	Evaluating the Robustness of Micro-Pillar Array Columns for High-Throughput Proteomics
Description	Nanoflow liquid chromatography capable of delivering consistent and reliable results across extensive sample sets is essential for the advancement of mass spectrometry-based proteomics. Micro-Pillar Array Columns (μPACs) represent a significant breakthrough, offering durability and performance stability. Here, we evaluate the robustness of μPACs by comparing a column used continuously for over 16 months with more than 7,000 injections (μPAC1) to a nearly new column (μPAC2). Analysis of two TMT-labeled yeast TKO standards (TKOpro10u, a 10-plex unit-resolved standard; and TKOpro12, a 12-plex isotopolog-inclusive standard) showed that μPAC1 and μPAC2 yielded comparable numbers of unique peptides and proteins, exhibited similar reproducibility, and delivered equivalent chromatographic and spectral quality. Notably, these data showed that μPAC1 maintained high performance with minimal degradation of data quality, highlighting the exceptional durability of μPAC technology. These findings underscore that μPAC could contribute to reduced workflow disruptions in high-throughput analytical workflows, notably in proteomics workflows using mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2025-11-24
AnnouncementXML	Submission_2025-11-24_14:04:03.904.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-23 12:05:59	ID requested
⏵ 1	2025-11-24 14:04:04	announced

Dataset with its publication pending

submitter keyword: μPAC, Tandem Mass Tags, column durability, LC-MS/MS, chromatographic performance

Joao A. Paulo
contact affiliation	Cell Biology Harvard Medical School Boston, MA, USA
contact email	joao_paulo@post.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD067649
     1. Label: PRIDE project
     2. Name: Evaluating the Robustness of Micro-Pillar Array Columns for High-Throughput Proteomics