⮝ Full datasets listing
PXD067503
PXD067503 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of Difficult-to-Remove Host Cell Proteins in Adeno-Associated Virus Downstream Processing |
| Description | A scalable, serotype-agnostic, and fully chromatographic downstream platform was developed for purification of adeno-associated virus (AAV) based on affinity (AC), ion-exchange (AEX), and multi-modal (MMC) chromatography. Quantitative proteomic profiling across each purification stage was performed using sequential window acquisition of all theoretical fragment ion mass spectra (SWATH-MS) to characterize residual host cell protein (HCP) retention for four AAV serotypes (AAV2, -5, -8, and -9) produced with suspension HEK293 cells. The downstream process showed cumulative vector genome (VG) yields ranging from 44.6 – 69.2% with full capsid enrichments ranging from 2.62 – 5.93-fold across all AAV samples. 880, 99, and 21 HCPs were identified in all AAV samples after AC, AEX, and MMC, respectively. The characterization of HCP retention described here advances the understanding of process-related impurity clearance in scalable AAV downstream systems, with implications for both purification process design and AAV interactions with host cell factors. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-11-15 |
| AnnouncementXML | Submission_2025-11-14_07:00:05.127.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lie Min |
| SpeciesList | scientific name: cellular organisms; NCBI TaxID: 131567; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide |
| Instrument | instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-19 07:15:09 | ID requested | |
| ⏵ 1 | 2025-11-14 07:00:05 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Host cell proteins (HCPs), Adeno-associated virus (AAV), Chromatography, Liquid chromatography-tandem mass spectrometry (LC-MS/MS), Difficult-to-remove, Downstream processing |
Contact List
| Kelvin H. Lee | |
|---|---|
| lab head | |
| Lie Min | |
| contact affiliation | University of Delware |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004016/ |
