PXD067489

PXD067489 is an original dataset announced via ProteomeXchange.

Title	Exploring Potential Mechanistic Aspects of "Biaoben acupoint " acupuncture for Lumbar Disc Herniation using 4D Proteomics
Description	Objective To investigate the mechanism of action of "Biaoben acupoint " acupuncture in treating Lumbar Disc Herniation (LDH) by identifying differentially expressed plasma proteins (DEPs) using timsTOF Pro-based 4D Data-Independent Acquisition (DIA) proteomics and correlating them with clinical indicators. Methods This study enrolled 10 healthy subjects (healthy group) and 10 patients with LDH (experimental group). The experimental group received "Biaoben acupoint" acupuncture treatment for 3 weeks. Clinical outcomes, including Visual Analogue Scale (VAS) for pain, Oswestry Disability Index (ODI) for lumbar function, and Japanese Orthopaedic Association (JOA) score for neurological status, were assessed before and after treatment. Plasma samples were collected for proteomic analysis. Results Acupuncture treatment significantly improved VAS, ODI, and JOA scores in the experimental group compared to baseline (p<0.001). Proteomic analysis quantified 3685 proteins, identifying 376 DEPs across the healthy group and the experimental group (before and after treatment). Bioinformatics analysis revealed that these DEPs were primarily enriched in pathways related to cell structure and adhesion (e.g., cytoskeleton remodeling, focal adhesion), inflammation and immune signaling (e.g., chemokine and cytokine signaling), and cell signal transduction (e.g., calcium signaling, Rap1 pathway). Core DEPs included ACTB, CXCR4, ACTN1, CXCL12, SELP, and CCN2. Correlation analysis demonstrated that the expression levels of CXCL12, ACTN1, CXCR4, and CCN2 were significantly correlated with VAS, ODI, and JOA scores. Conclusion "Biaoben acupoint" acupuncture likely exerts its therapeutic effects by modulating multiple biological pathways related to inflammatory/immune responses, cytoskeleton organization, cell structure/adhesion, and tissue repair, thereby improving pain, lumbar function, and neurological deficits in LDH patients. Proteins such as CXCL12, ACTN1, CXCR4, and CCN2 are potential key mediators of these therapeutic effects.
HostingRepository	iProX
AnnounceDate	2025-08-19
AnnouncementXML	Submission_2025-08-18_19:18:17.365.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fudong Shi
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-18 19:16:31	ID requested
⏵ 1	2025-08-18 19:18:17	announced

Dataset with its publication pending

submitter keyword: Lumbar Disc Herniation, Biaoben acupoint,Acupuncture,4D-DIA,quantitative proteomics,Differentially expressed proteins

Shimin Zhang
contact affiliation	China Academy of Chinese Medical Sciences,Wangjing Hospital
contact email	smzhang1117@163.com
lab head
Fudong Shi
contact affiliation	China Academy of Chinese Medical Sciences,Wangjing Hospital
contact email	shifudong929@163.com
dataset submitter

iProX dataset URI