⮝ Full datasets listing
PXD067244
PXD067244 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | EIF5A couples translational control with transcriptional reprogramming through chromocenter reorganization during spermiogenesis |
| Description | Translation initiation factor eIF5A facilitates protein synthesis and impacts diverse biological processes, yet its role in transcriptional regulation is poorly understood. Here we find eIF5A highly expressed in diverse spermatogenic cell types. Conditional knockout of Eif5a (SKO) causes complete infertility in male mice due to round spermatid arrest. Interestingly, eIF5A deletion severely compromises chromocenter integrity in round spermatids. Proteomic profiling reveals widespread dysregulation in eIF5A-deficient round spermatids, downregulated proteins are enriched for chromatin-associated functions, likely contributing to chromocenter dysfunction. Notably, ATAC-seq analysis shows increased chromatin accessibility upon eIF5A depletion, accompanied by transcriptional dysregulation of genes critical for acrosome and manchette formation. Our data underscore that eIF5A not only regulates the translation of chromatin-organizing proteins required for chromocenter stability but also influences transcriptional regulation by modulating chromatin landscape. These findings illuminate a novel, germ cell-specific pathway coupling translational control and transcriptional regulation via chromatin reorganization. |
| HostingRepository | iProX |
| AnnounceDate | 2025-08-12 |
| AnnouncementXML | Submission_2026-02-01_21:56:23.677.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuling Cai |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-11 20:08:43 | ID requested | |
| ⏵ 1 | 2026-02-01 21:56:24 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: eIF5A,spermatogenesis,male infertility,chromatin accessibility,translational-transcriptional coupling |
Contact List
| Hongbin Liu | |
|---|---|
| contact affiliation | Shandong University |
| contact email | hongbin_sduivf@aliyun.com |
| lab head | |
| Yuling Cai | |
| contact affiliation | Shandong University |
| contact email | caiyuling37@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
