PXD067162

PXD067162 is an original dataset announced via ProteomeXchange.

Title	Proteome of Anoplocephala perfoliata elucidate lineage-specific features of a “neglected” equine tapeworm
Description	Anoplocephala perfoliata is the most prevalent and pathogenic tapeworm (cestode) of horses worldwide, yet it remains molecularly understudied. Here, we present a chromosome-scale nuclear genome and matched proteomes for this parasite, establishing the first high-resolution molecular resource for the family Anoplocephalidae. The nuclear genome is 372.3 Mb in size and encodes 9,711 protein-coding genes, 78.2% of which were functionally annotated and ~80% supported by transcriptomic evidence. Proteomic analysis confirmed 758 proteins in previously-analysed excretory/secretory products from adult worms, including highly expressed components of the ubiquitin–proteasome system, stress response families – e.g., translationally controlled tumour protein (TCTP) and universal stress protein (USP) – and cytoskeletal scaffolds. Approximately 6.5% of the genome consisted of retroelements, predominantly LINEs. Comparative genomic analyses revealed a relatively conserved synteny with members of the family Taeniidae (Taenia multiceps, Echinococcus granulosus and E. multilocularis) and a pronounced structural divergence from Hymenolepis microstoma (Hymenolepididae), reflecting mosaic genome evolution within the order Cyclophyllidea. Classification of proteins inferred from the genome identified GTPases, kinases peptidases and secretome-associated proteins among the most abundant groups. A subset of proteins exhibited signal peptides or extracellular localisation, suggesting their role as parasite-derived proteins (PDPs) involved in host–parasite communication and immune evasion. This integrated genomic and proteomic framework reveals lineage-specific molecular adaptations in A. perfoliata and provides a foundation for future functional and translational investigations of this and closely related cestodes.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-15_14:40:49.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tao Wang
SpeciesList	scientific name: Anoplocephala perfoliata; NCBI TaxID: NEWT:218192;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2025-08-10 17:01:37	ID requested
⏵ 1	2025-12-15 14:40:49	announced

Dataset with its publication pending

submitter keyword: Anoplocephalidae,Anoplocephala perfoliata, LC-MS/MS

Robin Beat Gasser
contact affiliation	Melbourne Veterinary School, The University of Melbourne, Parkville, Victoria 3010, Australia
contact email	robinbg@unimelb.edu.au
lab head
Tao Wang
contact affiliation	The University of Melbourne
contact email	tao.wang1@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD067162

PRIDE project URI

• PRIDE
  1. PXD067162
     1. Label: PRIDE project
     2. Name: Proteome of Anoplocephala perfoliata elucidate lineage-specific features of a “neglected” equine tapeworm