PXD066956
PXD066956 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | TMT proteome analysis of mouse pancreatic tissue |
Description | Pancreatic tissues were collected from mice that had received 2 weeks of chronic treatment. After anesthetized with isoflurane, tissues were snap-frozen in liquid nitrogen and stored at -80℃. Protein concentration was determined, and then 100 μL 0.1 M TEAB buffer and 41 μL TMT labeling reagent (dissolved in acetonitrile) were added to the samples. After a 2-hour incubation, the reaction was terminated by adding 8% ammonia water. The labeled samples were combined in equal volumes, desalted, and lyophilized. The lyophilized samples were resuspended in mobile phase A (2% acetonitrile, pH adjusted to 10.0 with ammonium hydroxide) and subjected to gradient elution using mobile phase B (98% acetonitrile). Samples were centrifuged at 14,000 g for 20 min at 4℃. For analysis, an L-3000 HPLC system equipped with a Water BEH C18 column (4.6 × 250 mm, 5 μm) was used, with the column maintained at 45 °C. Secondary mass spectrometry, was performed using a Q ExactiveTM series mass spectrometer. The resolution was set to 45000 (at 200 m/z), the maximum capacity of C-trap was 5 × 104, and the maximum C-trap injection time was 86 ms. Peptide fragmentation was achieved using a 32% collision energy, with a threshold intensity of 1.2 × 105. |
HostingRepository | iProX |
AnnounceDate | 2025-08-05 |
AnnouncementXML | Submission_2025-08-04_20:15:25.636.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Feng Hong |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-08-04 20:15:05 | ID requested | |
⏵ 1 | 2025-08-04 20:15:26 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: TMT proteome, mouse, pancrease |
Contact List
Zuxin Chen | |
---|---|
contact affiliation | Shenzhen Institute of Advanced Technology |
contact email | zx.chen3@siat.ac.cn |
lab head | |
Feng Hong | |
contact affiliation | Shenzhen Institute of Advanced Technology |
contact email | hongfengshengli@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |