PXD066949
PXD066949 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Overexpression of the signaling-coordinator GAB2 can play an important role in Acute Myeloid Leukemia progression |
| Description | Tandem-mass-tag (TMT) proteomics experimental data from a study of murine acute myeloid leukemia (AML) that spontaneously develops in mice with Dnmt3aR878H/+ x Npm1cA/+ bone marrow. These mice spontaneously develop myelomonocytic AML in 6-15 months. We characterized pre-leukemic bone marrow samples, and 11 independent spontaneous AMLs that developed in these doubly-mutant mice ("mAML1-11").10 of 11 AMLs contained an amplification of murine chromosome 7 (chr7), usually as the only structural variant. Furthermore, we identified two non-leukemic mice where whole genome sequencing revealed amplification of chromosome 7 (+7) and no other cooperating mutations in identifiable driver genes (pre-tumor, +7 only). One of these clonal expansions was tested twice in secondary transplant assays, and did not commonly yield fatal disease when transplanted into recipients, suggesting that +7 is an intermediate step in AML progression. Further experiments identified Gab2 as an important gene on chromosome 7 for AML pathogenesis in this model. Retroviral overexpression of Gab2 in Dnmt3aR878H/+ x Npm1cA/+ bone marrow led to expansion of bone marrow cells both in vitro and in vivo in transplanted, recipient mice, as well as to accelerated development of AML; the resulting AML samples are called GAB2 mAML in this dataset. Measurements were performed from bulk bone marrow of mice. Data can be explored further at mAML.leylab.org. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-08-04 |
| AnnouncementXML | Submission_2025-08-04_15:14:16.738.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sai Mukund Ramakrishnan |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Carbamidomethyl; Oxidation; Acetyl; Pyro-carbamidomethyl; Glu->pyro-Glu; Phospho |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-08-04 14:27:53 | ID requested | |
| ⏵ 1 | 2025-08-04 15:14:17 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: AML, acute myeloid leukemia, DatasetType:Proteomics |
Contact List
| Timothy J. Ley | |
|---|---|
| contact affiliation | Washington University in St. Louis |
| contact email | timley@wustl.edu |
| lab head | |
| Sai Mukund Ramakrishnan | |
| contact affiliation | Washington University in St Louis |
| contact email | saimukund@wustl.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098719/ |
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