PXD066373

PXD066373 is an original dataset announced via ProteomeXchange.

Title	Comparative Proteomics Identified Proteins iMung Bean SproutsUnder Different Concentrations of Urea
Description	Mung bean (Vigna radiate) sprouts are a popular choice among sprouted vegetables in Asia. Currently, the impact of nitrogen sources on the growth of mung bean sprouts remains poorly understood, and the underlying biological mechanisms responsible for the observed nonlinear growth patterns at different nitrogen levels have yet to be elucidated. In this research, in addition to conventional growth monitoring and quality evaluation, a comparative proteomics method was applied to investigate the molecular mechanisms of mung bean in response to 0, 0.025, 0.05, 0.075, and 0.1% urea concentrations.Our results indicated that mung bean sprout height and yield increased with rising urea concentrations but were suppressed beyond theL3 level (0.075% urea). Nitrate nitrogen and free amino acid content rose steadily with urea levels, whereas protein content, nitrate reductase activity, and nitrite levels followed apeak-then-decline trend, peaking at intermediate concentrations. Differential expression protein analysis was conducted on mung bean sprouts treated with different concentrations of urea, and more differentially expressed proteins participated in the L3 urea concentration. Analysis of common differential proteins among comparison groups showed that the mung bean sprouts enhanced their adaptability to urea stress environments by upregulating chlorophyll a-b binding protein and cationic amino acid transporter and downregulating the levels of glycosyltransferase, L-ascorbic acid, and cytochrome P450.Theproteomic analysis uncovered the regulatory mechanisms governing these metabolic pathways, identifying 47 differentially expressed proteins (DEPs) involved in the biosynthesis of proteins, free amino acids, and nitrogen-related metabolites.
HostingRepository	iProX
AnnounceDate	2025-07-22
AnnouncementXML	Submission_2025-07-21_20:22:40.972.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Wei
SpeciesList	scientific name: Vigna radiata; NCBI TaxID: 157791;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-21 20:22:24	ID requested
⏵ 1	2025-07-21 20:22:41	announced

Dataset with its publication pending

submitter keyword: comparative proteomics

mung bean

urea

Xiaohan Liang
contact affiliation	Institute of Food Testing, Hainan Academy of Inspection andTesting
contact email	lxhan.88@163.com
lab head
Jing Wei
contact affiliation	Institute of Food Testing, Hainan Academy of Inspection andTesting
contact email	weijing0128@126.com
dataset submitter

iProX dataset URI