PXD065682

PXD065682 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Phosphorylated filamin-A at Serine 1459 from plasma-derived small extracellular vesicles as a promising biomarker for high-risk adenoma and colorectal cancer
Description	Parallel proteomic and phosphoproteomic analyses were conducted on plasma-derived small extracellular vesicles (sEVs) obtained from healthy individuals with negative colonoscopy (N), patients with advanced precancerous polyps (high-risk adenoma; HRA), and patients with colorectal cancer (CRC). In the discovery cohort, plasma samples were processed for sEV isolation using size-exclusion chromatography. The sEV isolates were digested with mass spectrometry-grade trypsin, and the resulting peptides were quantified using the Pierce Quantitative Colorimetric Peptide Assay. Peptides from each group (n = 10) were pooled, and 100 µg of the pooled peptides were aliquoted for phosphopeptide enrichment using the ProteoExtract Phosphopeptide Enrichment TiO2 Kit. Both the enriched phosphopeptides and corresponding unenriched peptides were analyzed in parallel using label-free quantitative mass spectrometry in three technical replicates. Findings were validated in individual samples (n = 50) through immunoblot analysis in the validation cohort.
HostingRepository	jPOST
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-06_01:57:42.609.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chris
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; N6-acetyl-L-lysine; alpha-amino acetylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-02 02:08:32	ID requested
⏵ 1	2026-04-06 01:57:43	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: phosphoproteomics
small extracellular vesicles
high-risk adenoma
colorectal cancer
filamin-A

submitter keyword: phosphoproteomics

small extracellular vesicles

high-risk adenoma

colorectal cancer

filamin-A

Contact List

(1) Dr. Churat Weeraphan, (2) Assoc. Prof. Dr. Supinya Thanapongpichat and (3) Assoc. Prof. Dr. Hansuk Buncherd
lab head
Chris
contact affiliation	Chulabhorn Research Institute
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003905/