PXD065676

PXD065676 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial components secretion in extracellular vesicles promotes alveolar epithelial mitochondrial quality control
Description	The injury of type 2 alveolar epithelial cells (AEC2s) is the core of the pathological processes responsible for idiopathic pulmonary fibrosis (IPF). AEC2s from IPF patients have been shown to exhibit mitochondrial dysfunction. However, the mechanisms that regulate AEC2 mitochondrial homeostasis remain poorly understood. Here, we report a pivotal role of G protein-coupled receptor class C group 5 member A (GPRC5A) in mitochondrial quality control in AEC2s through promoting mitochondrial secretion in extracellular vesicles (EVs). Through analyzing exon sequencing data from clinical cohort studies, we found that GPRC5A variants could be involved in IPF pathogenesis. In accordance with clinical data, mice lacking GPRC5A in AEC2s aggravates bleomycin-induced pulmonary fibrosis. We further demonstrate that GPRC5A deficiency in AEC2s reduces secretion of mitochondria in EVs and disrupts mitochondrial functions both in vitro and in vivo. Mechanistically, we determine that the GPRC5A-MIRO2 pathway facilitates the transfer of mitochondrial fragments into late endosomes. Collectively, our findings provide evidence of the shedding of mitochondrial components dependent on GPRC5A as a pathway of mitochondrial quality control in AEC2s, which is crucial in the maintenance of epithelial physiological activities and lung tissue homeostasis.
HostingRepository	PRIDE
AnnounceDate	2025-11-15
AnnouncementXML	Submission_2025-11-14_16:38:45.918.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YUE HAN
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-01 20:53:07	ID requested
⏵ 1	2025-11-14 16:38:46	announced

Dataset with its publication pending

submitter keyword: None

Yue Han
contact affiliation	School of Medicine, Chinese University of Hong Kong, Shenzhen
contact email	18704622690@163.com
lab head
YUE HAN
contact affiliation	Beihang University
contact email	814157218@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065676
     1. Label: PRIDE project
     2. Name: Mitochondrial components secretion in extracellular vesicles promotes alveolar epithelial mitochondrial quality control