PXD065659 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Polyamine-dependent metabolic shielding regulates alternative splicing |
| Description | Metabolites are central to cellular homeostasis. Whereas much emphasis has been placed on their relevance to meet energetic and biosynthetic demands, metabolic intermediates also function as signaling molecules. Here we show that polyamines, small polycations that are essential for cell biology, regulate the process of alternative pre-mRNA splicing. We find that the inhibition of polyamine synthesis increases the phosphorylation of spliceosomal proteins, concomitant with remarkable perturbation of alternative splicing in cells and tissues. Mechanistically, molecular modeling together with biochemical assays revealed that polyamines bind to acidic phosphorylatable motifs in splicing factors of the U2 snRNP SF3 subcomplex, thus preventing the action of kinases. The molecular process through which polyamines regulate protein phosphorylation is a phenomenon that we define as “metabolic shielding”. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-11-11 |
| AnnouncementXML | Submission_2025-11-11_02:36:24.867.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Maria Sendino |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-07-01 08:30:18 | ID requested | |
| ⏵ 1 | 2025-11-11 02:36:25 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Polyamine, splicing, phosphorylation |
Contact List
| Javier Muñoz |
|---|
| contact affiliation | IIS Biobizkaia |
| contact email | javier.munozperalta@bio-bizkaia.eus |
| lab head | |
| Maria Sendino |
|---|
| contact affiliation | Biobizkaia HRI |
| contact email | maria.sendinomouliet@bio-bizkaia.eus |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD065659
- Label: PRIDE project
- Name: Polyamine-dependent metabolic shielding regulates alternative splicing
