PXD065347

PXD065347 is an original dataset announced via ProteomeXchange.

Title	Astral-based DIA proteomics analysis reveals the effects of selenium on Pleurotus pulmonarius
Description	his study investigates the effects of sodium selenite on the yield, selenium content, and proteomic profile of Pleurotus pulmonarius (P. pulmonarius). Treatment with 2 μg/mL sodium selenite (PP-Se2) resulted in a 17.87% increase in the yield of P. pulmonarius. In contrast, treatment with 10 μg/mL sodium selenate (PP-Se10) caused a 23.58% reduction in yield. The selenium content exhibited a positive correlation with the sodium selenite concentration in the cultivation substrate. Based on data-independent acquisition (DIA) proteomics analysis, it was revealed that PP-Se2 and PP-Se10 contained 141 differential expressed proteins (DEPs) (64 up-regulated and 77 down-regulated) and 129 DEPs (73 up-regulated and 56 down-regulated), respectively. DEPs in PP-Se2 were predominantly involved in carbon metabolism, ribosomes, peroxisomes, and ABC transporters. In contrast, DEPs in PP-Se10 were primarily enriched in the biosynthesis of secondary metabolites and cysteine and methionine metabolism. These results suggest that P. pulmonarius exhibits distinct metabolic response mechanisms to varying levels of selenium treatment, with these pathways potentially being influenced by selenium concentration. These findings help to fill the gap in selenium proteomics research in edible fungi and provide new theoretical insights for the development of selenium-enriched functional foods.
HostingRepository	PRIDE
AnnounceDate	2025-07-22
AnnouncementXML	Submission_2025-07-22_05:23:53.064.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yujun Sun
SpeciesList	scientific name: Pleurotus pulmonarius; NCBI TaxID: 28995;
ModificationList	No PTMs are included in the dataset
Instrument	Thermo Fisher Scientific instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-23 09:19:38	ID requested
⏵ 1	2025-07-22 05:23:53	announced

Dataset with its publication pending

submitter keyword: Pleurotus pulmonarius

Sodium selenite

Proteomics

Selenium stress

Yujun Sun
contact affiliation	College of Biomedicine and Health, Anhui Science and Technology University, Fengyang, 233100, China
contact email	sunyj@ahstu.edu.cn
lab head
Yujun Sun
contact affiliation	corresponding author
contact email	sunyj@ahstu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD065347

PRIDE project URI

• PRIDE
  1. PXD065347
     1. Label: PRIDE project
     2. Name: Astral-based DIA proteomics analysis reveals the effects of selenium on Pleurotus pulmonarius