PXD064939

PXD064939 is an original dataset announced via ProteomeXchange.

Title	Autoimmunity-associated DIORA1 binds the MRCK family of serine/threonine kinases and controls cell motility
Description	Genetic association links DIORA1 to numerous autoimmune rheumatic diseases including systemic lupus erythematous, Sjögren's disease, myositis, scleroderma and rheumatoid arthritis, but its cellular function remains unknown. Here, we identify the MRCK kinase family as DIORA1 interactors. MRCK kinases regulate the activity of actin-myosin networks, including cell adhesion, motility and invasion. Mapping the interaction between DIORA1 and MRCKs, we found that DIORA1 binds three distinct modules of MRCK kinases, including the conserved autoinhibitory kinase inhibitory motif (KIM) and C1-PH-CNH domains. Knockdown of DIORA1 altered cellular phosphorylation patterns, including reduced phosphorylation of known MRCK targets. Functional analyses revealed enhanced cellular migration and invasion following knockdown of DIORA1, and RNA-sequencing and proteomic analyses confirmed upregulation of epithelial mesenchymal transition genes and proteins. In summary, our findings identify autoimmunity-associated DIORA1 as a previously uncharacterized interactor of MRCK kinases and a modulator of cell motility.
HostingRepository	PRIDE
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-08_14:16:36.266.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akos Vegvari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-12 07:03:53	ID requested
⏵ 1	2025-09-08 14:16:36	announced

Dataset with its publication pending

submitter keyword: Autoimmunity, Epithelial-mesenchymal transition (EMT), Rho GTPase signaling, DIORA1, MRCK kinases, Kinase inhibitory motif (KIM), Cancer, Cell motility, Cytoskeleton, FAM167A

Akos Vegvari
contact affiliation	Karolinska Institutet, Stockholm, Sweden
contact email	akos.vegvari@ki.se
lab head
Akos Vegvari
contact affiliation	Karolinska Institutet
contact email	akos.vegvari@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD064939

PRIDE project URI

• PRIDE
  1. PXD064939
     1. Label: PRIDE project
     2. Name: Autoimmunity-associated DIORA1 binds the MRCK family of serine/threonine kinases and controls cell motility