PXD064571

PXD064571 is an original dataset announced via ProteomeXchange.

Title	Radical footprinting in mammalian whole blood
Description	Hydroxyl Radical Protein Footprinting (HRPF) is a powerful tool to probe protein higher-order structure, as well as protein-protein and protein-carbohydrate interactions. It is mostly performed in vitro, but recent advances have extended its use to live cells, nematodes, and 3D cultures. However, application in living mammalian tissues has not been accomplished. Here, we present the first successful use of radical protein footprinting (RPF) in mammalian whole blood from wild-type (WT) and type 2 diabetes mellitus (T2DM) BKS. Cg Dock7m +/+ Leprdb/J mice. Using persulfate photoactivated with the FOX Photolysis System, we achieved effective protein labeling without significant disruption to blood cell morphology. An optimized quenching protocol eliminated background labeling. We report oxidative modifications in 11 selected proteins, revealing disease-associated conformational changes in multiple proteins. These findings demonstrate the feasibility of RPF in mammalian blood and open new opportunities for structural proteomics in preclinical models and clinical samples.
HostingRepository	PRIDE
AnnounceDate	2026-01-13
AnnouncementXML	Submission_2026-01-13_10:37:54.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandeep Misra
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-03 09:48:02	ID requested
⏵ 1	2026-01-13 10:37:55	announced

Dataset with its publication pending

submitter keyword: HRPF, targeted analysis, mouse blood, diabetes mellitus

Joshua S. Sharp
contact affiliation	University of Mississippi
contact email	skmisra@olemiss.edu
lab head
Sandeep Misra
contact affiliation	University of Mississippi
contact email	skmisra@olemiss.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD064571
     1. Label: PRIDE project
     2. Name: Radical footprinting in mammalian whole blood