PXD064403

PXD064403 is an original dataset announced via ProteomeXchange.

Title	Fungal CeRNase T2 from Cunninghamella echinulata disrupts detoxification pathways and cause behavioral inhibition in invasive ant, Solenopsis invicta (Buren)
Description	Solenopsis invicta known as one of the most dangerous invasive species due to its severe impacts on the human health, agricultural systems and biodiversity. This study investigates the insecticidal potential of Cunninghamella echinulata-derived proteins, focusing on the RNase T2 enzyme (CeRNase T2), against S. invicta. Crude protein extracts and purified CeRNase T2 were evaluated through bioassays, proteomics, and detoxification enzyme activity assays. CeRNase T2 exhibited superior toxicity, with 72 h LC50 (50% lethal concentration) values of 13.94 mg/L compared to 243.31 mg/L for crude extracts. At 250 mg/L, CeRNase T2 induced 100% mortality within 72 h, accompanied by complete loss of aggregation, climbing, and locomotion behaviors. Proteomic profiling revealed CeRNase T2’s structural stability (30.32 kDa, pI 7.57) and secretion via an N-terminal signal peptide. Mechanistically, CeRNase T2 disrupted redox homeostasis, elevating superoxide dismutase (21.3 U/mL, 1.4× control), peroxidase (22.6 U/mL, 4.3×), and catalase (61 U/mL, 5.8×) activities while suppressing glutathione S-transferase and cytochrome P450. Metabolomic analysis identified 672 differentially expressed metabolites in treated ants, including dysregulated purine/nucleotide metabolism and FoxO signaling intermediates linked to energy collapse and DNA damage. Behavioral assays confirmed dose-dependent impairment of aggression-related traits (e.g., 71.6% vs. 98.3% control aggregation at 12.5 mg/L). These findings establish CeRNase T2 as a multi-target mycoinsecticide, combining rapid lethality with metabolic disruption, and propose its integration into sustainable fire ant management.
HostingRepository	iProX
AnnounceDate	2025-05-29
AnnouncementXML	Submission_2025-05-29_00:28:47.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shini Li
SpeciesList	scientific name: Cunninghamella echinulata; NCBI TaxID: 76405;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-29 00:27:21	ID requested
⏵ 1	2025-05-29 00:28:47	announced

Dataset with its publication pending

submitter keyword: Cunninghamella echinulata

Shini Li
contact affiliation	Yunnan Agricultural University
contact email	lishini06@163.com
lab head
Shini Li
contact affiliation	Yunnan Agricultural University
contact email	lishini06@163.com
dataset submitter

iProX dataset URI