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PXD064328
PXD064328 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Modulation of Core-Fucosylation Oxidation for Selective Enrichment and Characterization of Cell Surface Glycoprotein |
| Description | Protein core-fucosylation plays a crucial role in regulating cell surface protein functions and is involved in various biological processes, including cell signaling, immune response, and cancer progression. However, core-fucosylation (CF) poses particular challenges in identification and characterization due to its low abundance and the high heterogeneity of N-glycosylation. To overcome these challenges, this study systematically investigated the oxidation mechanisms of core-fucose and developed a selective enrichment strategy that combines chemical oxidation with glycan truncation for cell surface core-fucosylation characterization. Specifically, sodium periodate was employed to selectively oxidize cell surface glycans. In combination with the endoglycosidases Endo M and Endo F3, which possessed complementary substrate specificity and broad tolerance, this approach efficiently truncated N-glycans and leaving core-fucosylated glycopeptides bearing aldehyde tags. Utilizing reversible hydrazide chemistry, core-fucosylated glycopeptides were selectively enriched. The developed strategy was applied to profile cell surface core-fucosylatd protein in HeLa cells, which yielded 74 core-fucosylated glycopeptides corresponding to 21 key cell surface drug targets, thereby validating the efficacy of this approach. Collectively, this study systematically investigatd the mechnism of core-fucosylation oxidation and developed a new technical tool for studying cell surface protein core-fucosylation. |
| HostingRepository | jPOST |
| AnnounceDate | 2025-12-09 |
| AnnouncementXML | Submission_2025-12-08_07:00:05.097.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xin Guo |
| SpeciesList | scientific name: 智人（人类）; NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; unknown modification; unknown modification |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-05-27 03:32:02 | ID requested | |
| ⏵ 1 | 2025-12-08 07:00:05 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Core-fucosylation, Oxidation, hydrazide chemistry |
Contact List
| Xin Guo | |
|---|---|
| lab head | |
| Xin Guo | |
| contact affiliation | Dalian Institute of Chemical Physics, Chinese Academy of Sciences |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003832/ |
