PXD064137

PXD064137 is an original dataset announced via ProteomeXchange.

Title	The citrullinating enzyme PADI4 governs progenitor cell proliferation and translation in developing hair follicles
Description	Posttranslational protein modifications have emerged as a mechanism regulating progenitor cell state transitions during tissue formation. Herein, we exploit the stereotyped hair follicle development to delineate the function of PADI4; an enzyme converting peptidylarginine to citrulline. Single cell-sequencing places Padi4 in both progenitor and differentiated hair lineage cells and indicate that PADI4 acts to repress transcription during hair follicle development. We establish PADI4 as a negative regulator of proliferation, acting on LEF1-positive hair shaft committed progenitor cells. Mechanistically, PADI4 citrullinates proteins associated with mRNA-processing and ribosomal biogenesis, and lack of PADI4 promotes protein synthesis and rRNA transcription in vivo. Characterizing key translational effectors, we demonstrate that PADI4 citrullinates the translational repressor 4E-BP1 and reveal a crosstalk between PADI4 activity and 4E-BP1 phosphorylation. This work sheds new light on how posttranslational modifications impact progenitor cell states and tissue formation.
HostingRepository	PRIDE
AnnounceDate	2025-07-28
AnnouncementXML	Submission_2025-07-28_03:05:45.861.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Genander
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-21 03:20:54	ID requested
⏵ 1	2025-07-28 03:05:46	announced

Dataset with its publication pending

submitter keyword: mouse, hair follicle, citrullination, PADI4

Maria Genander
contact affiliation	Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden
contact email	maria.genander@ki.se
lab head
Maria Genander
contact affiliation	Department of Cell and Molecular Biology Karolinska Institutet
contact email	maria.genander@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD064137

PRIDE project URI

• PRIDE
  1. PXD064137
     1. Label: PRIDE project
     2. Name: The citrullinating enzyme PADI4 governs progenitor cell proliferation and translation in developing hair follicles