PXD063787

PXD063787 is an original dataset announced via ProteomeXchange.

Title	TAG-PL: A Universal Proximity Labeling Platform for Mapping Mitochondrial Proteomes and Organelle Interactions Under Stress
Description	Mitochondrial dysfunction induces numerous diseases, yet current proximity labeling methods require gene transfection and membrane potential-sensitive probes, limiting their use in hard-to-transfect cells and disease models. We developed TAG-PL (Tailored Antibody-Guided Proximity Labeling), a transfection-free approach for in-depth mapping of mitochondrial proteome, achieving > 90% specificity and identifying > 450 mitochondrial proteins—more the coverage of existing non-transfection methods. Applied to heat-stressed macrophages, TAG-PL revealed dynamic mitochondrial proteome remodeling, including antioxidant responses and metabolic shifts during heat stress. Notably, we discovered physical interactions between stress granules and mitochondria, identifying 10 interaction mediators (including MSRA and UBA1). These findings establish stress granules as regulatory hubs for organelle dynamics and immune responses. TAG-PL’s high performance and broad applicability across diverse sample types, particularly immune cells and tissues, make it a powerful tool for dissecting mitochondrial function in disease models without genetic manipulation.
HostingRepository	jPOST
AnnounceDate	2025-12-22
AnnouncementXML	Submission_2025-12-21_17:43:49.051.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enming Miao
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-08 21:53:03	ID requested
⏵ 1	2025-12-21 17:43:49	announced

Dataset with its publication pending

submitter keyword: Mitochondrial proteome, Antibody-guided proximity labeling, SG-mitochondria interaction

Mingliang Ye, Hongqiang Qin, Enming Miao
lab head
Enming Miao
contact affiliation	Dalian Institute of Chemical Physics Chinese Academy of Sciences
dataset submitter

jPOST dataset URI

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