PXD063617

PXD063617 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative Extracellular Vesicle Proteomics by Multiplexed Data-Independent Acquisition
Description	This study established an optimized dimethyl labeling-based multiplexed DIA (mDIA) pipeline for quantitative proteomics of extracellular vesicles (EVs). Benchmarking different library generation strategies and software suites demonstrated superior performance of mDIA using project-specific libraries generated from small-scale StageTip fractionation. This approach enabled robust profiling of low-abundance EV proteins and successfully revealed proteomic changes associated with IDH1 mutation and inhibitor treatment in intrahepatic cholangiocarcinoma (iCCA) cell-derived EVs.
HostingRepository	jPOST
AnnounceDate	2025-05-05
AnnouncementXML	Submission_2025-05-05_12:55:21.258.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi-Kai Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-05 12:54:44	ID requested
⏵ 1	2025-05-05 12:55:21	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Extracellular vesicles, Proteomics, StageTip-based fractionation, mDIA, Intrahepatic cholangiocarcinoma, IDH1 mutation, AG-120

submitter keyword: Extracellular vesicles, Proteomics, StageTip-based fractionation, mDIA, Intrahepatic cholangiocarcinoma, IDH1 mutation, AG-120

Contact List

W. Andy Tao
lab head
Yi-Kai Liu
contact affiliation	Purdue University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003750/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003750/