PXD063500

PXD063500 is an original dataset announced via ProteomeXchange.

Title	Alpha-synuclein interacts with regulators of ATP homeostasis in mitochondria
Description	Mitochondrial dysfunction and accumulation of α-synuclein aggregates are hallmarks of the neurodegenerative Parkinson’s disease and may be interconnected. In order to investigate the interplay between α-synuclein and brain mitochondria at near atomic structural level we applied NMR and identified α-synuclein protein interactors using limited proteolysis-coupled mass spectrometry (LiP-MS). Several of the proteins identified were related to ATP synthesis and homeostasis and included subunits of ATP synthase and the adenylate kinase AK2. Furthermore, our data suggest that α-synuclein interacts with the Parkinson’s disease related protein DJ1. NMR analysis demonstrated that both AK2 and DJ1 bind to the C-terminus and other segments of α-synuclein. Using a functional assay for AK2, we showed that monomeric α-synuclein has an activating effect, whereas C-terminally truncated α-synuclein and α-synuclein in an amyloid fibrillar state had no significant effect on AK2 activity. Our results suggest that α-synuclein modulates ATP homeostasis in a manner dependent on its conformation and its C-terminal acidic segment.
HostingRepository	PRIDE
AnnounceDate	2025-07-19
AnnouncementXML	Submission_2025-07-18_20:00:50.181.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tetiana Serdiuk
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-30 07:14:55	ID requested
⏵ 1	2025-07-18 20:00:50	announced

Dataset with its publication pending

submitter keyword: LiP-MS, mitochondria,Alpha-synuclein

Paola Picotti
contact affiliation	Institute of Molecular Systems Biology, ETH Zurich
contact email	picotti@imsb.biol.ethz.ch
lab head
Tetiana Serdiuk
contact affiliation	Institute of Molecular Systems Biology, ETH Zurich, Switzerland
contact email	tserdiuk@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD063500

PRIDE project URI

• PRIDE
  1. PXD063500
     1. Label: PRIDE project
     2. Name: Alpha-synuclein interacts with regulators of ATP homeostasis in mitochondria