PXD063345

PXD063345 is an original dataset announced via ProteomeXchange.

Title	A phosphorelay circuit drives extracellular alkalinization in receptor kinase-mediated immune and cell wall damage signaling
Description	Extracellular alkalinization has long been recognized as a hallmark of plant cell-surface receptor activation, including during pattern-triggered immunity (PTI); yet the mechanisms driving elicitor-induced alkalinization and its role in plant signaling remain unclear. Here, we demonstrate that inhibition of autoinhibited H+-ATPases (AHAs) is required for elicitor-induced extracellular alkalinization. This alkalinization is essential for immune and cell wall damage signaling mediated by diverse plasma membrane-localised receptor kinases (RKs) likely through modulation of ligand-receptor interactions. Mechanistically, RKs transduce elicitor-triggered signaling via the receptor-like cytoplasmic kinase BOTRYTIS-INDUCED KINASE 1 (BIK1), which inhibits AHA activity by disrupting AHA-GENERAL REGULATORY FACTOR (GRF) interactions through a conserved phosphorylation event. This phosphorylation-driven extracellular alkalinization module is required for disease resistance and cell wall damage responses initiated by ligand-RK pairs. Our findings uncover a conserved phosphorelay circuit that broadly regulates extracellular alkalinization to coordinate RK signaling, illuminating a general mechanism for RK activation and stress resilience.
HostingRepository	PRIDE
AnnounceDate	2026-04-15
AnnouncementXML	Submission_2026-04-14_18:54:41.527.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FRank Menke
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-25 09:45:03	ID requested
⏵ 1	2026-04-14 18:54:42	announced

Dataset with its publication pending

submitter keyword: None

Frank Menke
contact affiliation	The Sainsbury Laboratory
contact email	Frank.Menke@tsl.ac.uk
lab head
FRank Menke
contact affiliation	the Sainsbury laboratory
contact email	frank.menke@tsl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063345
     1. Label: PRIDE project
     2. Name: A phosphorelay circuit drives extracellular alkalinization in receptor kinase-mediated immune and cell wall damage signaling