PXD063304

PXD063304 is an original dataset announced via ProteomeXchange.

Title	Post-translational modification of H2B C-terminal helix regulates nucleosome binding and chromatin signaling
Description	Histone H2B contains a highly conserved C-terminal (H2B C) helix that has been implicated in chromatin interactions and dynamics. The H2B C helix is positioned adjacent to a major site of nucleosome interactions called the acidic patch. Despite individual structural studies highlighting interactions between chromatin proteins and the H2B C helix, the general role of the helix in mediating nucleosome recognition has not been explored. Moreover, many post-translational modifications (PTMs) have been identified within the H2B C helix, but significant gaps exist in our understanding of their regulatory potential. In this study, we employed nucleosome affinity proteomics using a library of nucleosomes with mutations or PTMs of the H2B C helix to investigate contributions to nucleosome binding. Our work uncovers new spatial patterns of H2BC helix engagement across the proteome. We also demonstrate that H2BK120 ubiquitylation (H2BK120ub1) broadly disrupts nucleosome binding, phenocopying mutation of the acidic patch, while differentially regulating acidic patch-dependent chromatin functions. In contrast, lysine acetylation results in more subtle position-specific changes, highlighting a more general role of H2B C helix PTMs in tuning acidic patch recognition.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-25_07:29:44.685.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dennis Goldfarb
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-24 19:42:30	ID requested
⏵ 1	2025-08-25 07:29:45	announced

Dataset with its publication pending

submitter keyword: Nucleosomes, protein-protein interactions

Robert McGinty
contact affiliation	University of North Carolina, Chapel Hill, NC, USA
contact email	rmcginty@email.unc.edu
lab head
Dennis Goldfarb
contact affiliation	Cell Biology and Physiology Institute for Informatics
contact email	d.goldfarb@wustl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD063304
     1. Label: PRIDE project
     2. Name: Post-translational modification of H2B C-terminal helix regulates nucleosome binding and chromatin signaling