PXD063286

PXD063286 is an original dataset announced via ProteomeXchange.

Title	Proliferative proteome induced by HPV 16E6 and NFX1-123 partnership in longitudinal keratinocyte cultures
Description	Human papillomaviruses (HPV) cause cervical, anogenital, and oropharyngeal cancers. Despite the availability of preventive HPV vaccines, their poor uptake leaves individuals at risk for these cancers, many of which remain common globally, and others that are increasing domestically. The HPV 16 E6 (16E6) protein plays a significant role in inducing and maintaining cellular transformation of its infected host cell; however, 16E6 itself has no enzymatic activity and executes its functions through partnerships with host proteins. Previously, we demonstrated that 16E6 binds to the host protein NFX1-123, and NFX1-123 expression is increased in HPV 16 positive cervical cancer cell lines and primary cancers compared to normal tissues. In this study, we quantify growth patterns of 16E6-expressing keratinocytes with endogenous or overexpressed NFX1-123 (16E6/vec and 16E6/FN123, respectively) levels and interrogate proteome expression changes early and late in longitudinal growth cultures. Early passage 16E6/vec and 16E6/FN123 cells showed similar growth rates; however, late passage 16E6/FN123 cells had accelerated growth, greater population doublings, increased telomerase activity, and a dysplastic phenotype when compared to 16E6/vec cells. Matching this, mass spectrometry revealed only 22 differentially expressed proteins at early passage; meanwhile, late passaged cells had 827 differentially expressed protein among 16E6/FN123 and 16E6/vec cells. In that, DNA maintenance and repair, proteins namely MCM2 and MCM4, were highly expressed in the 16E6/FN123 compared to 16E6/vec cells. These findings indicate that the 16E6 and NFX1-123 partnership, especially with sustained higher levels of NFX1-123, alters the longitudinal cellular environment in a manner that may initiate a preneoplastic phenotype.
HostingRepository	MassIVE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-04_07:07:29.569.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emma Doud
SpeciesList	scientific name: Human papillomavirus; NCBI TaxID: 10566; scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	TMTpro; Oxidation
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-24 07:38:42	ID requested
⏵ 1	2025-08-04 07:07:29	announced

no publication

submitter keyword: Human papillomavirus (HPV), E6, NFX1-123, cervical cancer, DatasetType:Proteomics

Rachel Katzenellenbogen
contact affiliation	Indiana University School of Medicine
contact email	rkatzene@iu.edu
lab head
Amber L. Mosley
contact affiliation	Indiana University School of Medicine
contact email	almosley@iu.edu
lab head
Emma Doud
contact affiliation	IUSM
contact email	edoud@iu.edu
dataset submitter

MassIVE dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097715/