PXD063269

PXD063269 is an original dataset announced via ProteomeXchange.

Title	Thermal proteome profiling reveals meltome upon NLRP3 inflammasome activation
Description	NLR family pyrin domain containing 3 (NLRP3) involves in inflammasome complex assembly and innate immunity. Activation of the NLRP3 inflammasome induces conformational alterations in protein complexes, influencing their interactions with other molecules, which in turn affects protein thermal stability. To investigate the proteome-wide thermal stability alterations induced by NLRP3 inflammasome activation, we conducted a comprehensive analysis of meltome dynamics using thermal proteome profiling (TPP). Our analysis identified 337 proteins exhibiting alterations in thermal stability upon NLRP3 inflammasome activation. Subsequently, we validated three proteins by the cellular thermal shift assay (CETSA). Notably, our findings reveal that the majority of these proteins tend to cluster into distinct macromolecular complexes. Furthermore, we identify FAM120A as a novel NLRP3 binding partner, with its suppression enhancing caspase-1 activation and IL-1β release in response to NLRP3 agonist. Collectively, these data provide a comprehensive framework for understanding the mechanisms of NLRP3 inflammasome activation and underscore the utility of TPP in exploring proteome-wide thermal stability changes during signaling transduction.
HostingRepository	iProX
AnnounceDate	2025-04-24
AnnouncementXML	Submission_2025-04-24_00:21:10.095.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chaozhi Jin
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-24 00:20:46	ID requested
⏵ 1	2025-04-24 00:21:10	announced

Dataset with its publication pending

submitter keyword: NLRP3 Inflammasome activation, thermal proteome profiling, AP/MS, FAM120A

Jian Wang
contact affiliation	Beijing Proteome Research Center/ National Center for Protein Sciences(Beijing)
contact email	wangj.bio@foxmail.com
lab head
Chaozhi Jin
contact affiliation	Beijing Proteome Research Center/ National Center for Protein Sciences(Beijing)
contact email	jinx-1@163.com
dataset submitter

iProX dataset URI